Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 9 of 9

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Q81.
Which of the following is not included as a step for cloning mini Mu in vivo?

a.

A culture of target strain, whose DNA we want to clone is infected with mini Mu

b.

The phage is inserted at multiple sites in the genome

c.

Some of the packaged strain is flanked by two mini Mu genes

d.

The packaged phage is introduced into a second strain and Mu would replicate as a phage

Discuss
Answer: (d).The packaged phage is introduced into a second strain and Mu would replicate as a phage
Q82.
Which of the following is not included as a step in cloning in bacteriophage PI?

a.

The growth of vector is done as a plasmid by using pBR322 origin of replication

b.

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

c.

Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

d.

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

Discuss
Answer: (c).Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites
Q83.
What happens after packaging is carried out in the phage head?

a.

A sequence dependent cleavage of DNA is carried out

b.

A sequence independent cleavage of DNA is carried out

c.

They are linearized before they are infected into the bacterial cells

d.

They are circularized before they are infected into the bacterial cells

Discuss
Answer: (b).A sequence independent cleavage of DNA is carried out
Q84.
Circularization must take place in the bacterial cells before the replication starts.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q85.
How many origins of replication are there once the cyclization is carried out by loxP sites?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q86.
Choose the incorrect statement for PAC vectors.

a.

In these vectors, the phage packaging signals are removed but the two P1 origin of replication persist

b.

They resemble BAC vectors

c.

They accept an insert of upto 300 kbp

d.

Instability is observed

Discuss
Answer: (d).Instability is observed
Q87.
Choose the correct statement in respect to sacB gene?

a.

The cloning site for PAC lies on the side of sacB gene

b.

It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose

c.

Expression of sacB in the presence of sucrose is beneficial

d.

Levan is non-toxic for E. coli

Discuss
Answer: (b).It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose
Q88.
Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

a.

positive selection

b.

negative selection

c.

counter selection

d.

sacB selection

Discuss
Answer: (c).counter selection
Q89.
If recombination takes place between elements in the second strain, it would replicate by the origin of replication of mini Mu.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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