Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 3 of 9

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Q21.
The main advantage of phagemid is that it can be used for the generation of single or double stranded products without recloning.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q22.
It is very important to study lambda biology as lambda phages are used for cloning purposes. Which of the statement is true for lambda phage?

a.

It is an example of temperate phage

b.

The fate of the phage is decided before it infects the cell

c.

The lysis fate is that where the phage inserts its genome into the bacterial genome and the replication goes on

d.

The lysogenic fate is that where the phage infects the cell and lysis is carried out

Discuss
Answer: (a).It is an example of temperate phage
Q23.
At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.

a.

It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there

b.

In this method, the central portion is cut with another enzyme after being separated from the arms

c.

The arms are also cut with this second enzyme

d.

It is not necessary to remove the fragments generated

Discuss
Answer: (c).The arms are also cut with this second enzyme
Q24.
Physical separation is also used at times. Choose the correct statement with respect to this method.

a.

Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

b.

In sugar density gradient, the molecules are separated on the basis of density

c.

The central portion is recovered from the gel or the gradient

d.

Gel is more efficient than the sugar density gradient

Discuss
Answer: (a).Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient
Q25.
Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.

a.

Packaging in phage coat is a more efficient process than the normal transformation process

b.

The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration

c.

Lysate constitutes of lambda proteins needed for phage assembly

d.

The amount of non-recombinant phage in the background is less

Discuss
Answer: (d).The amount of non-recombinant phage in the background is less
Q26.
Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

a.

Separate strains with chain-termination mutations in different genes for coat components are used

b.

Temperature sensitive cI repressor is used

c.

Due to heat shock packaging proteins are induced

d.

Packaging is carried out in each strain

Discuss
Answer: (d).Packaging is carried out in each strain
Q27.
Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________

a.

A gene

b.

D gene

c.

E and D gene

d.

D and A gene

Discuss
Answer: (c).E and D gene
Q28.
Choose the correct statement for the infectious particle of lambda phage.

a.

It is single stranded genome

b.

It is circular double stranded genome

c.

The ends are blunt with cos (cohesive) sequences

d.

The ends are created by cleavage at cos sites during phage packaging

Discuss
Answer: (d).The ends are created by cleavage at cos sites during phage packaging
Q29.
Some changes are encountered in the infectious particle as it infects the cell. Choose the incorrect statement.

a.

The infectious particle circularizes upon causing infection by annealing at cos sites

b.

There are two promoters present, PR and PL

c.

Upon infection only PR is activated

d.

Promoters give rise to immediate early transcripts

Discuss
Answer: (c).Upon infection only PR is activated
Q30.
The immediate early transcripts direct synthesis of which genes?

a.

N genes

b.

cro genes

c.

Both N and cro genes

d.

PR and PL genes

Discuss
Answer: (c).Both N and cro genes
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