Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 3 of 9

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Q21.
Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________

a.

A gene

b.

D gene

c.

E and D gene

d.

D and A gene

Discuss
Answer: (c).E and D gene
Q22.
Which of the following is a restriction for cloning in bacteriophage Mu?

a.

It is limited to handling only small number of phage particles

b.

It relies on whether the DNA is flanked by two mini Mu genes or not

c.

Recombination between the elements in the second strain is not a restriction

d.

Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not

Discuss
Answer: (b).It relies on whether the DNA is flanked by two mini Mu genes or not
Q23.
What is the size of the insert that can be accommodated in the head of bacteriophage PI?

a.

30-40 kbp

b.

80-95 kbp

c.

110-115 kbp

d.

200-300 kbp

Discuss
Answer: (c).110-115 kbp
Q24.
What is the function of the pac site in the bacteriophage PI?

a.

It is responsible for the initiation of packaging

b.

It is responsible for the termination of packaging

c.

It is responsible for the initiation of replication

d.

It is responsible for the termination of replication

Discuss
Answer: (a).It is responsible for the initiation of packaging
Q25.
What is the function of loxP sites?

a.

It is responsible for linearization of DNA in the host bacterium

b.

It is responsible for circularization of DNA in the host bacterium

c.

It is responsible for conversion of single stranded DNA into double stranded DNA

d.

It is responsible for conversion of double stranded DNA into single stranded DNA

Discuss
Answer: (b).It is responsible for circularization of DNA in the host bacterium
Q26.
Which of the following is not included as a step in cloning in bacteriophage PI?

a.

The growth of vector is done as a plasmid by using pBR322 origin of replication

b.

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

c.

Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

d.

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

Discuss
Answer: (c).Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites
Q27.
What happens after packaging is carried out in the phage head?

a.

A sequence dependent cleavage of DNA is carried out

b.

A sequence independent cleavage of DNA is carried out

c.

They are linearized before they are infected into the bacterial cells

d.

They are circularized before they are infected into the bacterial cells

Discuss
Answer: (b).A sequence independent cleavage of DNA is carried out
Q28.
Circularization must take place in the bacterial cells before the replication starts.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q29.
Choose the correct statement for Sanger’s method of sequencing.

a.

They are also called as chain termination methods

b.

Use of M13 vector is made for getting double stranded DNA

c.

Use of dideoxynucleotide phosphate (ddNTP) is made

d.

The use of M13 vectors is more preferred these days

Discuss
Answer: (a).They are also called as chain termination methods
Q30.
Which of the statement doesn’t holds for phage display systems?

a.

The filamentous phage is often used as phage display system

b.

Coding sequences are inserted in one of the protein coating genes

c.

The most common protein coding genes are gene II or gene VIII

d.

The inserted is expressed along with other protein coat genes

Discuss
Answer: (c).The most common protein coding genes are gene II or gene VIII
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