Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 8 of 9

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Q71.
Which of the following is the correct method to check whether the DNA has entered into the cell or not in the case of cosmid?

a.

If a transformation has taken place turbid plaques are formed

b.

If a transformation has taken place clear plaques are formed

c.

If a transformation has taken place, it can be confirmed via ampicillin resistance

d.

If a transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also

Discuss
Answer: (c).If a transformation has taken place, it can be confirmed via ampicillin resistance
Q72.
Which size of the insert is accepted by the cosmids?

a.

10-20 kbp

b.

35-45 kbp

c.

50-60 kbp

d.

100-120 kbp

Discuss
Answer: (b).35-45 kbp
Q73.
What happens once the cosmid enters the E. coli cells?

a.

There is strict size selection inside E. coli cells

b.

Partial deletion may take place

c.

The tendency of deletion may is increased by using low copy number

d.

The tendency of deletion can’t be altered

Discuss
Answer: (b).Partial deletion may take place
Q74.
If colEI derived origin of replication is replaced by the origin of replication of F plasmid, it is called as:

a.

phagemid

b.

F cosmid

c.

plasmid

d.

fosmid

Discuss
Answer: (d).fosmid
Q75.
Bacteriophage Mu is also a phage, choose the correct statement for it.

a.

It is an example of temperate phage

b.

It is packaged into heads containing 50 kbp of DNA

c.

When the phage replicates it goes transposition only a few times

d.

Mu can’t be used for cloning in vivo

Discuss
Answer: (a).It is an example of temperate phage
Q76.
Choose the incorrect statement for ‘Mini Mu’?

a.

It is produced by cloning of bacteriophage Mu in vivo

b.

It contains reduced phage genome

c.

It doesn’t contain replication of origin of plasmid

d.

A selectable marker is added

Discuss
Answer: (c).It doesn’t contain replication of origin of plasmid
Q77.
Which of the following is not included as a step for cloning mini Mu in vivo?

a.

A culture of target strain, whose DNA we want to clone is infected with mini Mu

b.

The phage is inserted at multiple sites in the genome

c.

Some of the packaged strain is flanked by two mini Mu genes

d.

The packaged phage is introduced into a second strain and Mu would replicate as a phage

Discuss
Answer: (d).The packaged phage is introduced into a second strain and Mu would replicate as a phage
Q78.
Which of the following is a restriction for cloning in bacteriophage Mu?

a.

It is limited to handling only small number of phage particles

b.

It relies on whether the DNA is flanked by two mini Mu genes or not

c.

Recombination between the elements in the second strain is not a restriction

d.

Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not

Discuss
Answer: (b).It relies on whether the DNA is flanked by two mini Mu genes or not
Q79.
What is the size of the insert that can be accommodated in the head of bacteriophage PI?

a.

30-40 kbp

b.

80-95 kbp

c.

110-115 kbp

d.

200-300 kbp

Discuss
Answer: (c).110-115 kbp
Q80.
What is the function of the pac site in the bacteriophage PI?

a.

It is responsible for the initiation of packaging

b.

It is responsible for the termination of packaging

c.

It is responsible for the initiation of replication

d.

It is responsible for the termination of replication

Discuss
Answer: (a).It is responsible for the initiation of packaging
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