Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 4 of 9

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Q31.
M13 vector is tightly packed. Where is the available site to insert DNA?

a.

In the gene II

b.

Between gene II and IV

c.

Between gene III and V

d.

Between gene V and VIII

Discuss
Answer: (b).Between gene II and IV
Q32.
Choose the incorrect statement with respect to the collection of single stranded molecules.

a.

An infected culture is set up and after a while the cells are removed by centrifugation

b.

The phage is in the supernatant and is precipitated by addition of glycol and sodium chloride

c.

The phage pellet is resuspended and phenol is used for removal of protein

d.

The single stranded molecules sediment at the bottom

Discuss
Answer: (d).The single stranded molecules sediment at the bottom
Q33.
Choose the correct statement for Sanger’s method of sequencing.

a.

They are also called as chain termination methods

b.

Use of M13 vector is made for getting double stranded DNA

c.

Use of dideoxynucleotide phosphate (ddNTP) is made

d.

The use of M13 vectors is more preferred these days

Discuss
Answer: (a).They are also called as chain termination methods
Q34.
Which of the statement doesn’t holds for phage display systems?

a.

The filamentous phage is often used as phage display system

b.

Coding sequences are inserted in one of the protein coating genes

c.

The most common protein coding genes are gene II or gene VIII

d.

The inserted is expressed along with other protein coat genes

Discuss
Answer: (c).The most common protein coding genes are gene II or gene VIII
Q35.
Choose the correct statement for the synthesis of RNA probe?

a.

Double stranded DNA is only used for probe synthesis

b.

Single stranded DNA can be used for probe synthesis

c.

Probes are prepared for RNA transcripts which are specific for only particular strand

d.

Probes are prepared for RNA transcripts which are specific for both the strands

Discuss
Answer: (b).Single stranded DNA can be used for probe synthesis
Q36.
M13-plasmid vectors are often used. Which of the following is not a feature for this?

a.

They are known as phagemid, plage or phasmid

b.

Replication takes place through the origin of replication of M13 derivatives only

c.

Replication from M13 requires proteins to be provided by helper phage

d.

Some of the examples of these types of the combination are pUC 118, 119 and 120

Discuss
Answer: (b).Replication takes place through the origin of replication of M13 derivatives only
Q37.
The main advantage of phagemid is that it can be used for the generation of single or double stranded products without recloning.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q38.
It is very important to study lambda biology as lambda phages are used for cloning purposes. Which of the statement is true for lambda phage?

a.

It is an example of temperate phage

b.

The fate of the phage is decided before it infects the cell

c.

The lysis fate is that where the phage inserts its genome into the bacterial genome and the replication goes on

d.

The lysogenic fate is that where the phage infects the cell and lysis is carried out

Discuss
Answer: (a).It is an example of temperate phage
Q39.
Choose the correct statement for the infectious particle of lambda phage.

a.

It is single stranded genome

b.

It is circular double stranded genome

c.

The ends are blunt with cos (cohesive) sequences

d.

The ends are created by cleavage at cos sites during phage packaging

Discuss
Answer: (d).The ends are created by cleavage at cos sites during phage packaging
Q40.
Some changes are encountered in the infectious particle as it infects the cell. Choose the incorrect statement.

a.

The infectious particle circularizes upon causing infection by annealing at cos sites

b.

There are two promoters present, PR and PL

c.

Upon infection only PR is activated

d.

Promoters give rise to immediate early transcripts

Discuss
Answer: (c).Upon infection only PR is activated
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