Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 1 of 8

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Q1.
Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids?

a.

Bacterial plasmids are linear in nature

b.

They are single stranded

c.

Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property

d.

They are not capable of replication in bacteria

Discuss
Answer: (c).Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property
Q2.
Which of the following characteristic is not present in a plasmid on a general basis?

a.

Multiple cloning site (MCS)

b.

Origin of replication (ori)

c.

Antibiotic resistance gene

d.

Beta galactose genes

Discuss
Answer: (d).Beta galactose genes
Q3.
Bla is a gene, which is incorrect for it?

a.

It is an antibiotic resistance genes

b.

Antibiotic acts by blocking the cross-linking of the bacterial cell wall and thus leading to lysing of cells

c.

It encodes beta lactamase enzyme

d.

The beta lactam ring is activated

Discuss
Answer: (d).The beta lactam ring is activated
Q4.
Which of the characteristics is present in lacZ gene?

a.

It encodes for beta galactosidase enzyme

b.

Beta galactosidase enzyme is responsible for cleaving monosaccharides into the constituent elements

c.

It doesn’t cleaves a substrate called as X-gal

d.

But if X-gal is cleaved, it liberates pink coloured dye

Discuss
Answer: (a).It encodes for beta galactosidase enzyme
Q5.
Which of the following orientation is not propagated to later stages?

a.

Circular plasmids having genomic DNA

b.

Intramolecular ligation of plasmids

c.

Linear molecules

d.

Head to head ligation of two DNA plasmids

Discuss
Answer: (c).Linear molecules
Q6.
Molecules having new combination of sequences that were not present before are called as __________

a.

intermolecular ligants

b.

recombinants

c.

couple

d.

intramolecular ligants

Discuss
Answer: (b).recombinants
Q7.
Insertion of DNA into lacZ gene may lead to disruption of the gene function.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q8.
Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

a.

There are more chances of having two plasmids in a single cell

b.

There are more chances of having a single plasmid in one cell

c.

Uptake upto two plasmids is possible but not more than that

d.

Both are taken up with the same efficiency

Discuss
Answer: (b).There are more chances of having a single plasmid in one cell
Q9.
If genomic DNA has been inserted instead of the plasmid, what will happen?

a.

It would lead to inactivation of lacZ gene

b.

The X-gal substrate would be broken down

c.

The colonies formed are blue in colour

d.

The lacZ gene would be intact

Discuss
Answer: (a).It would lead to inactivation of lacZ gene
Q10.
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

a.

direct PCR

b.

colony PCR

c.

quantitative PCR

d.

in-situ PCR

Discuss
Answer: (b).colony PCR
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