Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 7 of 8

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Q61.
What happens if insert DNA is cut with two different restriction enzymes at the ends?

a.

It is difficult to insert the fragment

b.

The insert can be ligated in any orientation

c.

The insert can be ligated in one orientation only

d.

The amount of product increases

Discuss
Answer: (c).The insert can be ligated in one orientation only
Q62.
What is the disadvantage of amplification of using PCR over natural cloning?

a.

In PCR, there is no proof reading activity

b.

In PCR, small fragments can’t be amplified

c.

There is an A incorporated in PCR products, which makes cloning difficult

d.

PCR takes more time as compared to natural cloning

Discuss
Answer: (a).In PCR, there is no proof reading activity
Q63.
TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

a.

It is based on the fact that a T residue is incorporated at the end of the PCR product

b.

β€˜A’ residue is incorporated into the end of the vector

c.

It gives a low yield

d.

It is preferred over blunt end ligation

Discuss
Answer: (d).It is preferred over blunt end ligation
Q64.
Choose the incorrect statement in respect to topoisomerase I.

a.

It is used to cleave only one DNA strand

b.

It leaves at the recognition sequence –C/TCCTT- and is covalently attached to one end of the cleaved molecule

c.

The supercoiling of the DNA strand is altered and then it is sealed again by the enzyme

d.

It is slower than the normal DNA ligase

Discuss
Answer: (d).It is slower than the normal DNA ligase
Q65.
Linkers are often used in cloning. Choose the incorrect statement for linkers.

a.

These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence

b.

They are blunt ended molecules

c.

They are ligated to staggered ended insert molecules by T4 DNA ligase

d.

After treatment with enzyme, both the ends of the linker are staggered

Discuss
Answer: (c).They are ligated to staggered ended insert molecules by T4 DNA ligase
Q66.
There is no effect if the insert itself contains the restriction site.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q67.
Genes linked on E coli chromosome are

a.

lacZ

b.

lacY

c.

lacA

d.

all of above

Discuss
Answer: (d).all of above
Q68.
LacA encodes a detoxifying enzyme called

a.

Ξ²-galactoside transacetylase

b.

helicase

c.

polymerase

d.

gyrase

Discuss
Answer: (a).Ξ²-galactoside transacetylase
Q69.
The inhibition of gene expression by changing regulatory protein activity is termed as

a.

gene expression

b.

gene repression

c.

gene mutation

d.

gene localization

Discuss
Answer: (b).gene repression
Q70.
In E.coli the metabolism of lactose requires at least

a.

2 proteins

b.

3 proteins

c.

4 proteins

d.

5 proteins

Discuss
Answer: (a).2 proteins
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