Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 6 of 8

Discover more Topics under Genetic Engineering

Q51.
A collection of total genomic DNA from a single organism is called

a.

gene library

b.

gene cloning

c.

gene regulation

d.

gene expression

Discuss
Answer: (a).gene library
Q52.
The enzyme that cut the DNA at or near restriction sites are called as

a.

Nucleases

b.

Cutter

c.

Endonucleases

d.

restriction enzyme

Discuss
Answer: (d).restriction enzyme
Q53.
When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

a.

forced cloning

b.

orientation cloning

c.

correct cloning

d.

restricted cloning

Discuss
Answer: (a).forced cloning
Q54.
What happens if insert DNA is cut with two different restriction enzymes at the ends?

a.

It is difficult to insert the fragment

b.

The insert can be ligated in any orientation

c.

The insert can be ligated in one orientation only

d.

The amount of product increases

Discuss
Answer: (c).The insert can be ligated in one orientation only
Q55.
What is the disadvantage of amplification of using PCR over natural cloning?

a.

In PCR, there is no proof reading activity

b.

In PCR, small fragments can’t be amplified

c.

There is an A incorporated in PCR products, which makes cloning difficult

d.

PCR takes more time as compared to natural cloning

Discuss
Answer: (a).In PCR, there is no proof reading activity
Q56.
TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

a.

It is based on the fact that a T residue is incorporated at the end of the PCR product

b.

‘A’ residue is incorporated into the end of the vector

c.

It gives a low yield

d.

It is preferred over blunt end ligation

Discuss
Answer: (d).It is preferred over blunt end ligation
Q57.
The process in which the bacterial cell wall is disrupted by using small electric pulses is

a.

electroporation

b.

electric shock

c.

electric fragmentation

d.

electrolysis

Discuss
Answer: (a).electroporation
Q58.
Gene mapping provides useful information about chance of

a.

inheritance of disorders

b.

inheritance of genes

c.

inheritance of recessive gene

d.

inheritance of dominant gene

Discuss
Answer: (a).inheritance of disorders
Q59.
The methods used to identify the locus of the gene and distances between genes are called as

a.

gene localization

b.

gene linkage

c.

gene pool

d.

gene mapping

Discuss
Answer: (d).gene mapping
Q60.
The two basic rules of probability to solve problems in genetics include

a.

addition

b.

multiplication

c.

both a and b

d.

division

Discuss
Answer: (c).both a and b
Page 6 of 8