Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 3 of 8

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Q21.
It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________

a.

multiple cloning site

b.

origin of replication

c.

high copy number

d.

selectable marker

Discuss
Answer: (d).selectable marker
Q22.
Multiple cloning site (MCS) is defined as __________

a.

site within the plasmid which contains a site for many restriction enzymes

b.

site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid

c.

as the site containing many sites for only one restriction enzyme

d.

cloning many inserts together

Discuss
Answer: (b).site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid
Q23.
Size of the vector is related to having a suitable single restriction site.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q24.
If a restriction site is 6 nucleotides long, what are the chances of finding it in a vector?

a.

Once every 46 base pairs

b.

Once every 64 base pairs

c.

Once every 24 base pairs

d.

Once every 16 base pairs

Discuss
Answer: (a).Once every 46 base pairs
Q25.
Which of the statement is true for pBR322?

a.

It contains only an ampicillin resistance gene

b.

It contains both ampicillin resistant and tetracycline resistant gene

c.

The cloning site is present only in the ampicillin resistant gene

d.

It is a natural vector

Discuss
Answer: (b).It contains both ampicillin resistant and tetracycline resistant gene
Q26.
If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

a.

The insert is not present in any of the gene

b.

The insert is present in ampicillin gene but not in chloramphenicol gene

c.

The insert is present in choramphenicol gene but not in ampicillin gene

d.

The insert is present between both of the genes

Discuss
Answer: (c).The insert is present in choramphenicol gene but not in ampicillin gene
Q27.
If high copy number is there, the replication is called as ______ and if low copy number is there the replication is called as ______

a.

stringent, relaxed

b.

relaxed, stringent

c.

relaxed, relaxed

d.

stringent, stringent

Discuss
Answer: (a).stringent, relaxed
Q28.
Chloramphenicol amplification is carried out in various plasmids. Which of the statement is incorrect for it?

a.

It is used for increasing copy number in plasmids containing pMB1 origin

b.

Chloramphenicol promotes bacterial protein synthesis

c.

The protein synthesis is responsible for chromosomal DNA replication

d.

Chromosomal DNA replication is related to cell division

Discuss
Answer: (b).Chloramphenicol promotes bacterial protein synthesis
Q29.
The phenomenon of not allowing the recombinants to escape into the environment is termed as __________

a.

blocking

b.

termination

c.

disablement

d.

inactivation

Discuss
Answer: (c).disablement
Q30.
How will we choose which transformation method is to be used?

a.

It depends on the available conditions for the reaction to take place

b.

It depends on the efficiency which is required to be obtained

c.

All the transformation methods can be used equivalently

d.

Each reaction has been assigned a particular transformation method

Discuss
Answer: (b).It depends on the efficiency which is required to be obtained
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