Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 2 of 8

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Q11.
Insertion of DNA into lacZ gene may lead to disruption of the gene function.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q12.
Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

a.

There are more chances of having two plasmids in a single cell

b.

There are more chances of having a single plasmid in one cell

c.

Uptake upto two plasmids is possible but not more than that

d.

Both are taken up with the same efficiency

Discuss
Answer: (b).There are more chances of having a single plasmid in one cell
Q13.
If genomic DNA has been inserted instead of the plasmid, what will happen?

a.

It would lead to inactivation of lacZ gene

b.

The X-gal substrate would be broken down

c.

The colonies formed are blue in colour

d.

The lacZ gene would be intact

Discuss
Answer: (a).It would lead to inactivation of lacZ gene
Q14.
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

a.

direct PCR

b.

colony PCR

c.

quantitative PCR

d.

in-situ PCR

Discuss
Answer: (b).colony PCR
Q15.
Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

a.

genomic library

b.

screening

c.

recombination

d.

shotgun cloning

Discuss
Answer: (d).shotgun cloning
Q16.
The phenomenon of alpha complementation is __________

a.

Ξ± + Ξ² = Ο‰

b.

Ξ± = Ξ² + Ο‰

c.

Ξ² = Ξ± + Ο‰

d.

It is either Ξ± + Ξ² = Ο‰ or Ξ² = Ξ± + Ο‰

Discuss
Answer: (a).Ξ± + Ξ² = Ο‰
Q17.
An operon is defined as __________

a.

A related set of genes each having different promoters and are present differently

b.

A set of genes which are present together but are controlled by different promoters

c.

A set of genes which are present together and are controlled by the same promoter

d.

A set of genes which are not present together but controlled by the same promoter

Discuss
Answer: (c).A set of genes which are present together and are controlled by the same promoter
Q18.
Why the whole lacZ gene can’t be present in the plasmid at one time?

a.

Because the whole lacZ gene can’t be present anywhere

b.

The whole lac Z gene is very large in size and the plasmid size is small

c.

The whole lacZ gene is never functional

d.

Because the plasmid takes is having a restriction site only for taking up a portion of the lacZ gene

Discuss
Answer: (b).The whole lac Z gene is very large in size and the plasmid size is small
Q19.
Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it?

a.

One portion of the lacZ gene known as minigene is present in the plasmid

b.

Another portion is present in the host itself

c.

If they both are allowed to combine in the presence of IPTG, X-gal and ampicillin, blue colonies are observed

d.

If insert is also present along with host and plasmid, it results in the formation of blue colonies

Discuss
Answer: (d).If insert is also present along with host and plasmid, it results in the formation of blue colonies
Q20.
What will be the consequence of not having an origin of replication (ori) in the vector?

a.

If an ori is absent, replication of vector would not take place

b.

As the cells divide after taking up the vector, both the daughter cells would be having the vector

c.

A colony of transformed colonies is observed

d.

The vector won’t be taken up by the cell

Discuss
Answer: (a).If an ori is absent, replication of vector would not take place
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