Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 2 of 8

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Q11.
Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

a.

genomic library

b.

screening

c.

recombination

d.

shotgun cloning

Discuss
Answer: (d).shotgun cloning
Q12.
The phenomenon of alpha complementation is __________

a.

Ξ± + Ξ² = Ο‰

b.

Ξ± = Ξ² + Ο‰

c.

Ξ² = Ξ± + Ο‰

d.

It is either Ξ± + Ξ² = Ο‰ or Ξ² = Ξ± + Ο‰

Discuss
Answer: (a).Ξ± + Ξ² = Ο‰
Q13.
An operon is defined as __________

a.

A related set of genes each having different promoters and are present differently

b.

A set of genes which are present together but are controlled by different promoters

c.

A set of genes which are present together and are controlled by the same promoter

d.

A set of genes which are not present together but controlled by the same promoter

Discuss
Answer: (c).A set of genes which are present together and are controlled by the same promoter
Q14.
Why the whole lacZ gene can’t be present in the plasmid at one time?

a.

Because the whole lacZ gene can’t be present anywhere

b.

The whole lac Z gene is very large in size and the plasmid size is small

c.

The whole lacZ gene is never functional

d.

Because the plasmid takes is having a restriction site only for taking up a portion of the lacZ gene

Discuss
Answer: (b).The whole lac Z gene is very large in size and the plasmid size is small
Q15.
Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it?

a.

One portion of the lacZ gene known as minigene is present in the plasmid

b.

Another portion is present in the host itself

c.

If they both are allowed to combine in the presence of IPTG, X-gal and ampicillin, blue colonies are observed

d.

If insert is also present along with host and plasmid, it results in the formation of blue colonies

Discuss
Answer: (d).If insert is also present along with host and plasmid, it results in the formation of blue colonies
Q16.
What will be the consequence of not having an origin of replication (ori) in the vector?

a.

If an ori is absent, replication of vector would not take place

b.

As the cells divide after taking up the vector, both the daughter cells would be having the vector

c.

A colony of transformed colonies is observed

d.

The vector won’t be taken up by the cell

Discuss
Answer: (a).If an ori is absent, replication of vector would not take place
Q17.
Transformation efficiency is defined as __________

a.

ratio of transformed colonies by microgram of sample DNA that is to be inserted

b.

ratio of transformed colonies by amount of sample DNA that is to be inserted

c.

ratio of transformed colonies by microgram of plasmid DNA

d.

ratio of transformed colonies by amount of plasmid DNA

Discuss
Answer: (d).ratio of transformed colonies by amount of plasmid DNA
Q18.
After carrying out the cloning experiment, the cells are plated on agar. Along with agar, it also contains antibiotic resistant genes, X-gal and an inducer of lacZ gene. Which of the following would grow?

a.

Cells that have taken up plasmid DNA

b.

Cells that have taken up genomic DNA

c.

Cells having no insert

d.

Cells having no insert

Discuss
Answer: (a).Cells that have taken up plasmid DNA
Q19.
Choose the correct statement for adaptors.

a.

They are blunt ended at both the ends

b.

They are single stranded at both the ends

c.

They may be single stranded at one end and other end may be blunt

d.

They don’t have extra restriction sites within their sequence

Discuss
Answer: (c).They may be single stranded at one end and other end may be blunt
Q20.
It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________

a.

multiple cloning site

b.

origin of replication

c.

high copy number

d.

selectable marker

Discuss
Answer: (d).selectable marker
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