Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

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Polymerase Chain Reaction MCQs | Page 5 of 8

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Q41.
During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

a.

Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus

b.

Direct sequencing can be used if the template DNA is heterozygous at the locus

c.

If cloning is done before sequencing, then it is detected via using only a single clone for sequencing

d.

In the case several recombinants are used, it can’t go undetected

Discuss
Answer: (b).Direct sequencing can be used if the template DNA is heterozygous at the locus
Q42.
If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as ____________

a.

Heterzygosity

b.

Product heterogeneity

c.

Population heterogeneity

d.

Template heterogeneity

Discuss
Answer: (c).Population heterogeneity
Q43.
Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

a.

Amination of bases

b.

Chemical cross-linking between the strands

c.

Chemical cross-linking within the strands

d.

Slowing down the polymerase

Discuss
Answer: (a).Amination of bases
Q44.
If cytosine is deaminated, which of the base is formed?

a.

Thymine

b.

Guanine

c.

Adenine

d.

Uracil

Discuss
Answer: (d).Uracil
Q45.
Which of the statement is correct for misincorporation?

a.

If direct sequencing has carried this misincorporation is a great problem

b.

The erroneous molecules give strong signals than genuine molecules in case of misincorporation

c.

If the misincorporation in cloned PCR products it is a problem

d.

Even if the error is induced at an early stage it is not incorporated in many sequences

Discuss
Answer: (c).If the misincorporation in cloned PCR products it is a problem
Q46.
Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?

a.

The error caused because of polymerase is biased for the first position in the codon

b.

The error caused because of polymerase is evenly distributed on all the positions in the codon

c.

The error caused by sequence heterogeneity is mainly because of first position in the codon

d.

The error caused by sequence heterogeneity is evenly distributed on all the codon positions

Discuss
Answer: (b).The error caused because of polymerase is evenly distributed on all the positions in the codon
Q47.
Which of the statement is incorrect for jumping PCR?

a.

It is used in the case when the DNA fragment is degraded

b.

In this type of PCR, the molecules are not long enough to span between the two primer sites

c.

At the end of first round of synthesis, the extension of the molecule from the primer site to the end of the fragmented molecule takes place

d.

It doesn’t leads to the formation of chimeric product

Discuss
Answer: (d).It doesn’t leads to the formation of chimeric product
Q48.
What are the possibilities which can occur until the temperature has reached for primer annealing?

a.

Extension doesn’t starts until the appropriate temperature is reached

b.

Extension may start even when the temperature is low

c.

At low temperature, there is specific annealing of primer taking place

d.

There are more specific products which are generated

Discuss
Answer: (b).Extension may start even when the temperature is low
Q49.
Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

a.

The basis is that extension is not started until the first cycle reaches its maximum temperature

b.

The polymerase is added after the first cycle has reached its maximum temperature or melting temperature

c.

It is satisfactory for small number of samples

d.

It leads to generation of non specific products

Discuss
Answer: (d).It leads to generation of non specific products
Q50.
An alternative to adding polymerase at later stage is __________

a.

Make the polymerase inactive by binding it to an antibody

b.

Introduce the polymerase or Magnesium in clay beads

c.

Make the polymerase inactive by attaching groups which cause stearic inhindrance

d.

Introduction of polymerase or Magnesium in plastic wires

Discuss
Answer: (a).Make the polymerase inactive by binding it to an antibody
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