Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Polymerase Chain Reaction knowledge to the test? Let's get started with our carefully curated MCQs!

Polymerase Chain Reaction MCQs | Page 6 of 8

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Q51.
The primer annealing temperature is often very low from the maximum temperature. This low temperature leads to some mismatches.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q52.
Touch-down PCR is another modification. Its characteristics include:

a.

Lowering the temperature for primer annealing

b.

Primer annealing is done at higher temperatures initially

c.

The temperature is abruptly reduced in the second cycle

d.

In earlier cycles less stringent conditions are there and in later cycles, more stringent conditions are there

Discuss
Answer: (b).Primer annealing is done at higher temperatures initially
Q53.
If two successive PCR are carried out, it is called as __________

a.

Touch-down PCR

b.

Hot-start PCR

c.

Combined PCR

d.

Nested PCR

Discuss
Answer: (d).Nested PCR
Q54.
If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?

a.

First PCR

b.

Second PCR

c.

Both the PCRs

d.

It depends on the annealing temperature

Discuss
Answer: (a).First PCR
Q55.
The process of inserting an amplified PCR product in a vector for cloning is known as __________

a.

making library

b.

insertion

c.

making a hard copy

d.

making a PCR based vector

Discuss
Answer: (c).making a hard copy
Q56.
How can PCR product be cloned into a vector?

a.

It can be done only when PCR products are blunt-ended

b.

It can be done only by restriction enzyme digestion

c.

Both the methods can be used

d.

The blunt-ended approach is favoured

Discuss
Answer: (b).It can be done only by restriction enzyme digestion
Q57.
Which of the following statement is incorrect regarding the cloning of PCR products?

a.

In cloning via restriction enzymes, restriction sites are induced before amplification is carried out

b.

The restriction sites are induced in the primers before annealing

c.

The intermediate molecules are having restriction sites at both ends

d.

The amplified molecules can be cut at both the ends by appropriate enzymes

Discuss
Answer: (c).The intermediate molecules are having restriction sites at both ends
Q58.
Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

a.

The restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers

b.

The topoismerase I is used for cutting both the strands

c.

The induction of topoisomerase enzyme is done into the vector in the case it is very small in size

d.

The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

Discuss
Answer: (d).The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector
Q59.
Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________

a.

Inverse PCR

b.

Circular PCR

c.

Non-conventional PCR

d.

In-situ PCR

Discuss
Answer: (a).Inverse PCR
Q60.
Which one of the following is not done if amplification of the non flanking region is carried out?

a.

Firstly, restriction enzyme digestion is done of those sites whose sequence is not known

b.

Then the self-ligation of molecules is allowed

c.

Now the molecules are cleaved where the known sequence is

d.

Again, the molecules are linearized and the known sequence is in the middle

Discuss
Answer: (d).Again, the molecules are linearized and the known sequence is in the middle
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