Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Polymerase Chain Reaction knowledge to the test? Let's get started with our carefully curated MCQs!

Polymerase Chain Reaction MCQs | Page 4 of 8

Discover more Topics under Genetic Engineering

Q31.
Which of the statements don’t hold true for the forensics and the amplification carried out?

a.

In the case of forensics, conventional methods such as southern blotting are used very effectively

b.

In cases of bone fragments which contain less than 300 nucleotides conventional methods can’t be applied as they involve southern blotting, restriction digestion etc

c.

The poor condition of DNA also makes the PCR amplification difficult

d.

Microsatellites composed of simply varying repeats of CA sequences are used

Discuss
Answer: (a).In the case of forensics, conventional methods such as southern blotting are used very effectively
Q32.
The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________

a.

restriction fragment length polymorphism (RFLP)

b.

amplified fragment length polymorphism (AFLP)

c.

random amplification of polymorphic DNA (RAPD)

d.

polymorphism

Discuss
Answer: (c).random amplification of polymorphic DNA (RAPD)
Q33.
PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can’t be cultured axenically.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q34.
PCR amplification can be used for which type of samples?

a.

Old samples only

b.

Recent samples only

c.

Equally to both recent and old samples

d.

Recent samples are preferred but can be applied to old samples also

Discuss
Answer: (c).Equally to both recent and old samples
Q35.
What is the problem associated with historical DNA samples?

a.

They are less in amount thus amplification is difficult

b.

Because the samples are very old, there can be contamination

c.

They degrade during repeated cooling and heating cycles

d.

As the samples are old, the standard sequences for comparison is not present

Discuss
Answer: (b).Because the samples are very old, there can be contamination
Q36.
Which of the statement holds for long-range PCR and in its relation?

a.

It is the PCR in which longer templates are used

b.

DNA polymerases which don’t have proof-reading activity give larger products

c.

DNA polymerases’ processivity is not a measure to have larger products

d.

It is PCR in which a mixture of enzymes is used to have larger products

Discuss
Answer: (d).It is PCR in which a mixture of enzymes is used to have larger products
Q37.
Which of the following conditions don’t contribute to wrong annealing to primer?

a.

Chance complementarity

b.

Conditions of annealing

c.

The original sequence of the primers

d.

Both the conditions of annealing and the original sequence don’t play any role

Discuss
Answer: (d).Both the conditions of annealing and the original sequence don’t play any role
Q38.
How can the specificity of primer annealing be increased?

a.

Use of short primers

b.

Raising temperature

c.

Adjusting the concentration of sodium ions

d.

Using polymerase with proof reading activity

Discuss
Answer: (b).Raising temperature
Q39.
There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q40.
Which can be used as a precaution in order to minimize contamination?

a.

Careful use and design of pipettes

b.

Placing the pre-PCR and post-PCR stages in the same rooms

c.

Extracting the DNA along with surface layers

d.

Use of primers carefully is not very important

Discuss
Answer: (a).Careful use and design of pipettes
Page 4 of 8