Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Polymerase Chain Reaction knowledge to the test? Let's get started with our carefully curated MCQs!

Polymerase Chain Reaction MCQs | Page 2 of 8

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Q11.
What is the half life cycle for Taq polymerase?

a.

40 minutes

b.

80 minutes

c.

10 minutes

d.

50 minutes

Discuss
Answer: (a).40 minutes
Q12.
Taq polymerase incorporates which residue at 3’ end?

a.

G

b.

T

c.

A

d.

C

Discuss
Answer: (c).A
Q13.
Polymerases are also available from other Thermus species. Which of the following is correct?

a.

Thermus flavus gives Tfl enzyme

b.

Thermus thermopilus gives Tfl enzyme

c.

They are having proof reading activity

d.

Thermus flavus gives Tth enzyme

Discuss
Answer: (a).Thermus flavus gives Tfl enzyme
Q14.
Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?

a.

They add A residue at 3’ end

b.

They are obtained from Thermococcus litoralis

c.

They can’t be obtained from archaebacteria

d.

The marine bacteria from which they are obtained grow at temperatures lower than that of Thermus aquatics

Discuss
Answer: (b).They are obtained from Thermococcus litoralis
Q15.
Thermococcus litoralis grows at a temperature upto 98 degrees.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q16.
Primers are generally ____________

a.

20-30 nucleotides long

b.

40-50 nucleotides long

c.

as long as the template is

d.

taken according to the amount available

Discuss
Answer: (a).20-30 nucleotides long
Q17.
Which end of the primer should be matched properly in order to carry out the amplification?

a.

5’ end

b.

3’ end

c.

Both of the ends should be matched properly

d.

Anyone of the ends should match

Discuss
Answer: (b).3’ end
Q18.
Which of the following nucleotides should be there at 3’ end?

a.

Any of A, T, G or C will work out

b.

Either A or T

c.

Either G or C

d.

Specifically G

Discuss
Answer: (c).Either G or C
Q19.
Melting temperature is given by ____________

a.

4(G+C) + 2(A+T)

b.

2(G+C) + 4(A+T)

c.

2(A+G) + 4(C+T)

d.

4(A+G) + 2(C+T)

Discuss
Answer: (a).4(G+C) + 2(A+T)
Q20.
Both the primers, the start primer and the end primer should have a nearly same melting temperature.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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