Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 12 of 24

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Q111.
The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to the number of ____________

a.

sugar molecules

b.

nitrogenous bases

c.

phosphate groups

d.

both phosphate group and sugar molecules

Discuss
Answer: (c).phosphate groups
Q112.
Force is defined as mass per unit acceleration. As the number of phosphate molecules increases, the charge also increases which increases the force required. The acceleration is dependent on the size of the molecules.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q113.
A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?

a.

Filtration and centrifugation

b.

Filtration or centrifugation

c.

Allowing to sediment

d.

By passing through a silica column

Discuss
Answer: (b).Filtration or centrifugation
Q114.
Another method of DNA recovery is termed as freeze-squeeze. The correct statement for it is?

a.

The slice containing DNA is cut and frozen into liquid oxygen

b.

Freezing doesn’t have any effect on the DNA structure

c.

After freezing centrifugation is carried out through glass wool plug

d.

The substance used for centrifugation allows the gel to pass through but the liquid is retained

Discuss
Answer: (c).After freezing centrifugation is carried out through glass wool plug
Q115.
If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________

a.

secondary electrophoresis

b.

recovery electrophoresis

c.

denaturing electrophoresis

d.

electro-elution

Discuss
Answer: (d).electro-elution
Q116.
The DNA is sliced and is placed into the dialysis tube containing buffer. Further electrophoresis is performed. After it, the DNA moves out of the gel but is retained by buffer.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q117.
Often the DNA or RNA needs to be removed from the gel to the solid support. The process can be termed as ____________

a.

Blotting

b.

Transfer

c.

Chromatography

d.

DNA purification

Discuss
Answer: (a).Blotting
Q118.
Which of the following steps is not included in the transferring of DNA?

a.

The gel containing the DNA is immersed in the buffer in the reservoir

b.

The buffer flows up through the gel via a wick

c.

Membrane is placed over the gel

d.

The nucleic acids are dissolved in the buffer and are obtained from it via pipetting

Discuss
Answer: (d).The nucleic acids are dissolved in the buffer and are obtained from it via pipetting
Q119.
If the DNA samples are to be analyzed, the membrane should be studied. Which of them is correct for it?

a.

The membrane should not be treated with UV or heat

b.

The membrane is further immersed in the buffer containing labelled nucleic acids which are known as strips

c.

Labelled nucleic acids bind to the sequences which are same as the sequences present on the membrane

d.

The binded sequences can be visualized by autoradiography

Discuss
Answer: (d).The binded sequences can be visualized by autoradiography
Q120.
In the case DNA is transferred from gel to the membrane, it is known as ____________

a.

southern blot

b.

western blot

c.

northern blot

d.

south-western blot

Discuss
Answer: (a).southern blot
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