Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 8 of 24

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Q71.
In the case of blunt-end ligation, blunt ends can be generated by ___________

a.

simply the action of restriction endonuclease which gives straight ends

b.

the polishing of staggered ends

c.

both the action of restriction endonuclease which gives straight ends and polishing of staggered ends

d.

by the action of restriction endonuclease which gives staggered ends

Discuss
Answer: (c).both the action of restriction endonuclease which gives straight ends and polishing of staggered ends
Q72.
The ligation reaction is more efficient in which case?

a.

Blunt end ligation

b.

Sticky end ligation

c.

Both have the same efficiency

d.

Depends on the reaction conditions

Discuss
Answer: (b).Sticky end ligation
Q73.
The sticky ends are held together by which type of bonds?

a.

Hydrogen bond

b.

Covalent bond

c.

Ionic bond

d.

Van-der-waal forces

Discuss
Answer: (a).Hydrogen bond
Q74.
Why sticky ended ligations are carried out at temperatures lower than room temperature?

a.

It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends

b.

The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature

c.

The enzyme carrying out ligation is unstable at low temperature

d.

The sticky ends created, don’t just relegate at low temperature

Discuss
Answer: (a).It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends
Q75.
Ligation reaction can be both intramolecular and intermolecular in nature.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q76.
If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?

a.

Intramolecular

b.

Intermolecular

c.

Both observe recircularization equally

d.

Recirculation is not possible in any of the cases

Discuss
Answer: (a).Intramolecular
Q77.
What is the kinetics of the intramolecular and intermolecular ligation reactions?

a.

Second order kinetics for intramolecular and first order for intermolecular

b.

First order kinetics for intramolecular and second order for intermolecular

c.

Both are first order

d.

Both are second order

Discuss
Answer: (b).First order kinetics for intramolecular and second order for intermolecular
Q78.
What are the effects of increasing concentration of reaction components?

a.

It increases chances of ligation in both intramolecular and intermolecular reactions

b.

It increases chances of ligation only in intermolecular and no effect on intramolecular

c.

It decreases chances of ligation in intramolecular and increases in that of intermolecular

d.

It decreases chances of ligation in both types of reaction

Discuss
Answer: (b).It increases chances of ligation only in intermolecular and no effect on intramolecular
Q79.
Enzyme commonly used for carrying out ligation reaction is ____________

a.

Transferase

b.

Reverse transciptase

c.

Ligase

d.

DNase

Discuss
Answer: (c).Ligase
Q80.
Which of the following statements is correct with respect to T4 DNA ligase?

a.

It can carry out only blunt ended ligations

b.

It doesn’t requires ATP

c.

It requires a phosphate group at 3’ end and a hydroxyl group at 5’ end for the molecule to be joined

d.

It is obtained from T4 bacteriophage upon infection by E. coli

Discuss
Answer: (d).It is obtained from T4 bacteriophage upon infection by E. coli
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