Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 8 of 24

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Q71.
What is the function of methylase?

a.

Addition of methyl groups to DNA

b.

Removal of methyl groups from DNA

c.

Both in removal and addition of methyl groups from DNA

d.

It is used in production of methane gas

Discuss
Answer: (a).Addition of methyl groups to DNA
Q72.
Methylase is useful in cloning experiments.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q73.
Ligation is defined as ______________

a.

Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands

b.

Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands

c.

Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands

d.

Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

Discuss
Answer: (c).Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands
Q74.
If only one bond is broken in the sugar-phosphate backbone, it is called as ___________

a.

gap

b.

nick

c.

break

d.

leakage

Discuss
Answer: (b).nick
Q75.
How many categories of ligation reaction are there on the basis of ends created?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q76.
In the case of blunt-end ligation, blunt ends can be generated by ___________

a.

simply the action of restriction endonuclease which gives straight ends

b.

the polishing of staggered ends

c.

both the action of restriction endonuclease which gives straight ends and polishing of staggered ends

d.

by the action of restriction endonuclease which gives staggered ends

Discuss
Answer: (c).both the action of restriction endonuclease which gives straight ends and polishing of staggered ends
Q77.
The ligation reaction is more efficient in which case?

a.

Blunt end ligation

b.

Sticky end ligation

c.

Both have the same efficiency

d.

Depends on the reaction conditions

Discuss
Answer: (b).Sticky end ligation
Q78.
The sticky ends are held together by which type of bonds?

a.

Hydrogen bond

b.

Covalent bond

c.

Ionic bond

d.

Van-der-waal forces

Discuss
Answer: (a).Hydrogen bond
Q79.
Why sticky ended ligations are carried out at temperatures lower than room temperature?

a.

It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends

b.

The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature

c.

The enzyme carrying out ligation is unstable at low temperature

d.

The sticky ends created, don’t just relegate at low temperature

Discuss
Answer: (a).It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends
Q80.
Ligation reaction can be both intramolecular and intermolecular in nature.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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