Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Basics of Genetic Engineering. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Basics of Genetic Engineering knowledge to the test? Let's get started with our carefully curated MCQs!

Basics of Genetic Engineering MCQs | Page 6 of 24

Discover more Topics under Genetic Engineering

Q51.
How is phosphatase related to the ligation reactions?

a.

Phosphate group is not required for the ligation reaction to take place, thus phosphatase is helpful

b.

It is helpful in ceasing the unwanted ligation

c.

Phosphatases are not at all related to ligation reactions

d.

They act as a catalyst in case of the ligation reaction

Discuss
Answer: (b).It is helpful in ceasing the unwanted ligation
Q52.
How can phosphatase activity terminated prior to a ligation reaction?

a.

By heating

b.

By cooling

c.

By creating vibrations

d.

By alternatively cooling and heating

Discuss
Answer: (a).By heating
Q53.
Basic classification of polymerases includes how many types?

a.

2

b.

3

c.

4

d.

5

Discuss
Answer: (d).5
Q54.
Polymerase can be defined as ______________

a.

an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand

b.

an enzyme used for removal of nucleotides from the DNA or RNA strand

c.

an enzyme which can synthesize only a new DNA strand, not an RNA strand

d.

an enzyme which can synthesize either a new DNA or an RNA strand but only when a strand is there

Discuss
Answer: (a).an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand
Q55.
Which of the following activity is not possible in the case of DNA polymerase I?

a.

3’-5’ exonuclease

b.

5’-3’ exonuclease

c.

5’-3’ DNA synthesis

d.

3’-5’ DNA synthesis

Discuss
Answer: (d).3’-5’ DNA synthesis
Q56.
The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?

a.

The smaller fragment is having C terminal and the larger fragment is having N terminal

b.

The smaller fragment is named as Klenow fragment and the intact molecule is called as Kornberg fragment

c.

The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity

d.

Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity

Discuss
Answer: (c).The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity
Q57.
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

a.

polishing and end filling respectively

b.

end filling and polishing respectively

c.

polishing in both the cases

d.

end filling in both the cases

Discuss
Answer: (a).polishing and end filling respectively
Q58.
Thermostable DNA polymerases are very important in PCR. How are they obtained?

a.

They are obtained by heating the bacteria manually over high temperatures

b.

They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents

c.

They are found everywhere in nature

d.

They are obtained by genetically modifying the E. coli bacteria with thermal stability property

Discuss
Answer: (b).They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents
Q59.
Which of the following enzyme is said as reverse transcriptase?

a.

DNA dependent DNA polymerase

b.

RNA dependent RNA polymerase

c.

RNA dependent DNA polymerase

d.

DNA dependent RNA polymerase

Discuss
Answer: (c).RNA dependent DNA polymerase
Q60.
Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?

a.

Because they are not having 3’-5’ proofreading exonucleolytic activity

b.

Because they are not having 5’-3’ proofreading exonucleolytic activity

c.

Because they are having slow rates of exonucleolytic activity

d.

It is difficult to synthesize DNA from RNA

Discuss
Answer: (a).Because they are not having 3’-5’ proofreading exonucleolytic activity
Page 6 of 24