Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 6 of 24

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Q51.
UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it?

a.

In this method, the DNA bands are visualized because they fluoresce under UV

b.

The DNA bands won’t fluoresce but will absorb the UV light

c.

It is not suitable for visualizing single stranded molecules

d.

It is less preferred over the use of ethidium bromide

Discuss
Answer: (b).The DNA bands won’t fluoresce but will absorb the UV light
Q52.
The greatest separation is obtained in which portion of the gel?

a.

Lower portion where the anode is

b.

Lower portion where the cathode is

c.

The separation is uniform all over

d.

It varies according to the quantity of the size of the molecules to be separated

Discuss
Answer: (a).Lower portion where the anode is
Q53.
Which of the statements is correct for non uniform separation obtained other than the conventional one?

a.

It can be obtained via applying strong field towards the cathode

b.

It can be obtained via applying the same field on both the sides

c.

The separation is greater on the side of stronger field

d.

Its is greater towards the weaker field

Discuss
Answer: (c).The separation is greater on the side of stronger field
Q54.
How can gradient in the field strength be obtained?

a.

It can be obtained via varying the amount of current, keeping the resistance constant

b.

It can be obtained via varying the amount of resistance, keeping the current constant

c.

Both can be varied, but resistance is having more effect

d.

Buffer-gradient gels can be used with decreasing concentration of buffer at the bottom

Discuss
Answer: (b).It can be obtained via varying the amount of resistance, keeping the current constant
Q55.
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

a.

polishing and end filling respectively

b.

end filling and polishing respectively

c.

polishing in both the cases

d.

end filling in both the cases

Discuss
Answer: (a).polishing and end filling respectively
Q56.
Thermostable DNA polymerases are very important in PCR. How are they obtained?

a.

They are obtained by heating the bacteria manually over high temperatures

b.

They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents

c.

They are found everywhere in nature

d.

They are obtained by genetically modifying the E. coli bacteria with thermal stability property

Discuss
Answer: (b).They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents
Q57.
Which of the following enzyme is said as reverse transcriptase?

a.

DNA dependent DNA polymerase

b.

RNA dependent RNA polymerase

c.

RNA dependent DNA polymerase

d.

DNA dependent RNA polymerase

Discuss
Answer: (c).RNA dependent DNA polymerase
Q58.
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

a.

Wedge gels

b.

Buffer-gradient gels

c.

Pulse field gels

d.

Varying the amount of agarose will carry out the separation

Discuss
Answer: (c).Pulse field gels
Q59.
Which of the following statement is correct for the method of separation of large molecules?

a.

The large DNA molecules can pass through the matrix in any direction, not necessarily in the direction parallel to the movement

b.

The separation can be carried out if the fields are at right angles to each other

c.

The time of pulse should be less than that of the reorientation time

d.

Separation is not of the megabase level

Discuss
Answer: (b).The separation can be carried out if the fields are at right angles to each other
Q60.
The random change in allele frequencies is

a.

genetic drift

b.

diversity

c.

linkage

d.

Gene pool

Discuss
Answer: (a).genetic drift
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