Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 2 of 24

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Q11.
The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________

a.

reflects, can be determined

b.

reflects, can’t be determined

c.

doesn’t necessarily reflects, can be determined

d.

doesn’t necessarily reflects, can’t be determined

Discuss
Answer: (d).doesn’t necessarily reflects, can’t be determined
Q12.
For a convenient transformation system, _____ can be used for gene silencing.

a.

antisense RNA

b.

transposon insertion

c.

either antisense RNA or transposon insertion

d.

transposon insertion followed by antisense RNA

Discuss
Answer: (a).antisense RNA
Q13.
The term ‘endonuclease’ refers to cutting the DNA sequence from ______________

a.

only within the polynucleotide chain, not at the ends

b.

the ends of the chain

c.

anywhere in the chain

d.

exactly in the middle of the chain

Discuss
Answer: (a).only within the polynucleotide chain, not at the ends
Q14.
The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA?

a.

By methylation of bacterial DNA by restriction enzyme

b.

By methylation of foreign DNA by restriction enzyme

c.

By phosphorylation of bacterial DNA by restriction enzyme

d.

By phosphorylation of foreign DNA by restriction enzyme

Discuss
Answer: (a).By methylation of bacterial DNA by restriction enzyme
Q15.
Even after replication, how the modified DNA remains protected?

a.

It remains protected because of conservative mode of replication

b.

It remains protected because of semi-conservative mode of replication

c.

The mode of replication has no role to play in the protection

d.

It is again modified after replication

Discuss
Answer: (b).It remains protected because of semi-conservative mode of replication
Q16.
How many classes of restriction enzymes are there?

a.

2

b.

1

c.

3

d.

4

Discuss
Answer: (c).3
Q17.
Type II cuts the sequence in the following way __________

a.

Within the recognition sequence

b.

At 100-1000 nucleotides away from the recognition sequence

c.

At 27-30 nucleotides away from the recognition sequence

d.

It cuts randomly

Discuss
Answer: (a).Within the recognition sequence
Q18.
Which of the following is correct with respect to caesium chloride centrifugation?

a.

Caesium is light in weight

b.

The dissolution of caesium and nucleic acids leads to the formation of gradients

c.

According to the amount of supercoiling and A+T content, the DNA settles

d.

Nicked DNA settles below than supercoiled DNA

Discuss
Answer: (b).The dissolution of caesium and nucleic acids leads to the formation of gradients
Q19.
Which of the following components settles at the bottom?

a.

RNA

b.

Proteins

c.

Nicked DNA

d.

Supercoiled DNA

Discuss
Answer: (a).RNA
Q20.
The location of plasmid DNA can be visualized by addition of:

a.

bromophenol blue

b.

ethidium bromide

c.

ortho xylene

d.

texas red

Discuss
Answer: (b).ethidium bromide
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