Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 2 of 24

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Q11.
Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?

a.

Agarose is a polysaccharide which is obtained from red algae

b.

Agarose is a polysaccharide obtained from fungus

c.

It is composed of glucose residues

d.

It is obtained via dissolving in its water by boiling in water and then cooling it

Discuss
Answer: (a).Agarose is a polysaccharide which is obtained from red algae
Q12.
Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

a.

They are obtained via polymerization between acrylamide and bis-acrylamide

b.

The components added for initiating polymerization are ammonium persulphate and TEMED

c.

It is casted in horizontal and flat trays

d.

TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

Discuss
Answer: (c).It is casted in horizontal and flat trays
Q13.
If the amount of agarose added is more, the molecular under analysis should have the following characteristic:

a.

small size

b.

large size

c.

size has no relation with the amount of agarose

d.

the amount of molecules under analysis matters

Discuss
Answer: (a).small size
Q14.
In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?

a.

Renaturing

b.

Denaturing

c.

The routine agarose gel

d.

The routine polyacrylamide gel

Discuss
Answer: (b).Denaturing
Q15.
At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?

a.

The gelling temperature has an important role to play

b.

For higher gelling temperatures, digestion of DNA is done via the addition of agarase or some chaotropic agents

c.

For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting

d.

The DNA can be extracted via the addition of sodium or any positively charged group

Discuss
Answer: (d).The DNA can be extracted via the addition of sodium or any positively charged group
Q16.
UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it?

a.

In this method, the DNA bands are visualized because they fluoresce under UV

b.

The DNA bands won’t fluoresce but will absorb the UV light

c.

It is not suitable for visualizing single stranded molecules

d.

It is less preferred over the use of ethidium bromide

Discuss
Answer: (b).The DNA bands won’t fluoresce but will absorb the UV light
Q17.
The greatest separation is obtained in which portion of the gel?

a.

Lower portion where the anode is

b.

Lower portion where the cathode is

c.

The separation is uniform all over

d.

It varies according to the quantity of the size of the molecules to be separated

Discuss
Answer: (a).Lower portion where the anode is
Q18.
Which of the statements is correct for non uniform separation obtained other than the conventional one?

a.

It can be obtained via applying strong field towards the cathode

b.

It can be obtained via applying the same field on both the sides

c.

The separation is greater on the side of stronger field

d.

Its is greater towards the weaker field

Discuss
Answer: (c).The separation is greater on the side of stronger field
Q19.
How can gradient in the field strength be obtained?

a.

It can be obtained via varying the amount of current, keeping the resistance constant

b.

It can be obtained via varying the amount of resistance, keeping the current constant

c.

Both can be varied, but resistance is having more effect

d.

Buffer-gradient gels can be used with decreasing concentration of buffer at the bottom

Discuss
Answer: (b).It can be obtained via varying the amount of resistance, keeping the current constant
Q20.
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

a.

Wedge gels

b.

Buffer-gradient gels

c.

Pulse field gels

d.

Varying the amount of agarose will carry out the separation

Discuss
Answer: (c).Pulse field gels
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