Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 9 of 24

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Q81.
If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?

a.

Intramolecular

b.

Intermolecular

c.

Both observe recircularization equally

d.

Recirculation is not possible in any of the cases

Discuss
Answer: (a).Intramolecular
Q82.
What is the kinetics of the intramolecular and intermolecular ligation reactions?

a.

Second order kinetics for intramolecular and first order for intermolecular

b.

First order kinetics for intramolecular and second order for intermolecular

c.

Both are first order

d.

Both are second order

Discuss
Answer: (b).First order kinetics for intramolecular and second order for intermolecular
Q83.
What are the effects of increasing concentration of reaction components?

a.

It increases chances of ligation in both intramolecular and intermolecular reactions

b.

It increases chances of ligation only in intermolecular and no effect on intramolecular

c.

It decreases chances of ligation in intramolecular and increases in that of intermolecular

d.

It decreases chances of ligation in both types of reaction

Discuss
Answer: (b).It increases chances of ligation only in intermolecular and no effect on intramolecular
Q84.
Enzyme commonly used for carrying out ligation reaction is ____________

a.

Transferase

b.

Reverse transciptase

c.

Ligase

d.

DNase

Discuss
Answer: (c).Ligase
Q85.
Which of the following statements is correct with respect to T4 DNA ligase?

a.

It can carry out only blunt ended ligations

b.

It doesn’t requires ATP

c.

It requires a phosphate group at 3’ end and a hydroxyl group at 5’ end for the molecule to be joined

d.

It is obtained from T4 bacteriophage upon infection by E. coli

Discuss
Answer: (d).It is obtained from T4 bacteriophage upon infection by E. coli
Q86.
If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

a.

E. coli DNA ligase

b.

T4 DNA ligase

c.

Both of these enzymes act equally in carrying out blunt ended ligations

d.

None of them is able to carry out blunt ended ligations

Discuss
Answer: (b).T4 DNA ligase
Q87.
E. coli DNA ligase doesn’t require a cofactor.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q88.
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

a.

AMP is added to the 5’ phosphate of one of the DNA molecule

b.

It leads to the liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP

c.

The AMP is further displaced by an electrophilic attack

d.

The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule

Discuss
Answer: (a).AMP is added to the 5’ phosphate of one of the DNA molecule
Q89.
Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?

a.

They act only on double stranded molecules

b.

They alter the degree of supercoiling of DNA molecules

c.

They are less effective than conventional DNA ligase

d.

There are three types of topoisomerases

Discuss
Answer: (b).They alter the degree of supercoiling of DNA molecules
Q90.
Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________

a.

Ligase

b.

Transciptase

c.

Transposase

d.

Endonuclease

Discuss
Answer: (c).Transposase
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