Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 4 of 24

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Q31.
Why is DNase preferred over restriction endonuclease in some cases?

a.

DNase is preferred over restriction endonuclease in some cases because the latter are not able to recognize some of the restriction sites

b.

DNase is more specific as restriction endonuclease, so the required fragment is obtained

c.

DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments

d.

DNase is abundant in comparison the restriction endonuclease

Discuss
Answer: (c).DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments
Q32.
The ends created by use of DNase have unique single stranded sequences.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q33.
Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true?

a.

Sonication, needles, syringes, etc all come under the category of physical stress

b.

In physical stress, there are no chances of contamination

c.

The ends obtained have unique sequences

d.

They are less effective than DNase treatment

Discuss
Answer: (a).Sonication, needles, syringes, etc all come under the category of physical stress
Q34.
Phosphatases refer to __________

a.

the enzymes which add phosphate group at the end of the DNA molecule in the place of hydroxyl group

b.

the enzymes which hydrolytically remove phosphate group from the DNA molecules and replace them with hydroxyl group

c.

the enzymes responsible for removal of phosphate group from the DNA molecules and replace them with hydrogen

d.

the enzymes responsible for replacing hydrogen in the DNA molecules with the phosphate group

Discuss
Answer: (b).the enzymes which hydrolytically remove phosphate group from the DNA molecules and replace them with hydroxyl group
Q35.
How is phosphatase related to the ligation reactions?

a.

Phosphate group is not required for the ligation reaction to take place, thus phosphatase is helpful

b.

It is helpful in ceasing the unwanted ligation

c.

Phosphatases are not at all related to ligation reactions

d.

They act as a catalyst in case of the ligation reaction

Discuss
Answer: (b).It is helpful in ceasing the unwanted ligation
Q36.
How can phosphatase activity terminated prior to a ligation reaction?

a.

By heating

b.

By cooling

c.

By creating vibrations

d.

By alternatively cooling and heating

Discuss
Answer: (a).By heating
Q37.
Which of the following is correct with respect to caesium chloride centrifugation?

a.

Caesium is light in weight

b.

The dissolution of caesium and nucleic acids leads to the formation of gradients

c.

According to the amount of supercoiling and A+T content, the DNA settles

d.

Nicked DNA settles below than supercoiled DNA

Discuss
Answer: (b).The dissolution of caesium and nucleic acids leads to the formation of gradients
Q38.
Which of the following components settles at the bottom?

a.

RNA

b.

Proteins

c.

Nicked DNA

d.

Supercoiled DNA

Discuss
Answer: (a).RNA
Q39.
The location of plasmid DNA can be visualized by addition of:

a.

bromophenol blue

b.

ethidium bromide

c.

ortho xylene

d.

texas red

Discuss
Answer: (b).ethidium bromide
Q40.
Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?

a.

Circular

b.

Supercoiled

c.

Nicked

d.

Nicked

Discuss
Answer: (b).Supercoiled
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