Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 4 of 24

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Q31.
Blunt ends created by the restriction endonuclease can be joined.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q32.
The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it?

a.

The ends created are double stranded

b.

The single stranded end is 5’ in nature

c.

The single stranded end is 3’ in nature

d.

To decide about the nature of the ends more information is needed

Discuss
Answer: (b).The single stranded end is 5’ in nature
Q33.
The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?

a.

The ends created by both the enzymes are compatible

b.

The ends created by both the enzymes are not-compatible

c.

The ends created by DpnI are single stranded

d.

The ends created by Sau3A are single stranded

Discuss
Answer: (b).The ends created by both the enzymes are not-compatible
Q34.
The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it?

a.

The molecules which are cut by the same enzyme, anneal only if the sequence is palindromic in nature

b.

When the molecules are cleaved by the same enzyme and the recognition sequence is palindromic in nature, there is no effect on annealing

c.

There are increased chances of annealing if the recognition sequence is palindromic in nature

d.

The term ‘palindromic’ can be used whether the sequence is read from 5’ to 3’ or 3’ to 5’

Discuss
Answer: (c).There are increased chances of annealing if the recognition sequence is palindromic in nature
Q35.
If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif?

a.

1/256

b.

1/64

c.

1/16

d.

1/8

Discuss
Answer: (a).1/256
Q36.
Isoschizomers are defined as ___________

a.

enzymes having same recognition sequence and always cutting at the same site

b.

enzymes having same recognition sequence and always cutting at different site

c.

enzymes having different recognition site and cutting at the same site

d.

enzymes having same recognition site and they may or may not cut at the same site

Discuss
Answer: (d).enzymes having same recognition site and they may or may not cut at the same site
Q37.
Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?

a.

DpnI (GA|TC) and Sau3A (|GATC)

b.

BamHI (G|GATCC) and Sau3A (|GATC)

c.

DpnI (GA|TC) and BglII (A|GATCT)

d.

XbaI (T|CTAGA) and BamHI (G|GATCC)

Discuss
Answer: (b).BamHI (G|GATCC) and Sau3A (|GATC)
Q38.
The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:

a.

star activity

b.

specificity elevation

c.

concentration gradient effects

d.

diamond activity

Discuss
Answer: (a).star activity
Q39.
Which of the following statements is correct with respect to a unit of an enzyme?

a.

One unit of enzyme is defined as the amount of enzyme required to digest 1miligram of standard DNA in a specific time of 1hr and under given temperature conditions

b.

The amount of enzyme required doesn’t vary with the number of sites present in the DNA

c.

If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites

d.

The amount of enzyme required for digestion of DNA with less number of sites is more than that of more number of sites in the same amount of DNA

Discuss
Answer: (c).If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites
Q40.
Which of the following statement is correct regarding partial digestion?

a.

It is defined as the conditions where all the sites in the DNA sequence are not recognized

b.

The number of fragments created by partial digestion are same as that of complete digestion

c.

It is not useful in representation of genomic library

d.

Exactly half of the sites in the DNA are recognized

Discuss
Answer: (a).It is defined as the conditions where all the sites in the DNA sequence are not recognized
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