Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 5 of 24

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Q41.
Isoschizomers are defined as ___________

a.

enzymes having same recognition sequence and always cutting at the same site

b.

enzymes having same recognition sequence and always cutting at different site

c.

enzymes having different recognition site and cutting at the same site

d.

enzymes having same recognition site and they may or may not cut at the same site

Discuss
Answer: (d).enzymes having same recognition site and they may or may not cut at the same site
Q42.
Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?

a.

DpnI (GA|TC) and Sau3A (|GATC)

b.

BamHI (G|GATCC) and Sau3A (|GATC)

c.

DpnI (GA|TC) and BglII (A|GATCT)

d.

XbaI (T|CTAGA) and BamHI (G|GATCC)

Discuss
Answer: (b).BamHI (G|GATCC) and Sau3A (|GATC)
Q43.
The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:

a.

star activity

b.

specificity elevation

c.

concentration gradient effects

d.

diamond activity

Discuss
Answer: (a).star activity
Q44.
Which of the following statements is correct with respect to a unit of an enzyme?

a.

One unit of enzyme is defined as the amount of enzyme required to digest 1miligram of standard DNA in a specific time of 1hr and under given temperature conditions

b.

The amount of enzyme required doesn’t vary with the number of sites present in the DNA

c.

If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites

d.

The amount of enzyme required for digestion of DNA with less number of sites is more than that of more number of sites in the same amount of DNA

Discuss
Answer: (c).If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites
Q45.
Which of the following statement is correct regarding partial digestion?

a.

It is defined as the conditions where all the sites in the DNA sequence are not recognized

b.

The number of fragments created by partial digestion are same as that of complete digestion

c.

It is not useful in representation of genomic library

d.

Exactly half of the sites in the DNA are recognized

Discuss
Answer: (a).It is defined as the conditions where all the sites in the DNA sequence are not recognized
Q46.
Which of the following is the correct nomenclature of a restriction enzyme obtained from the first activity of strain R of Escherichia coli?

a.

EcoR1

b.

EscRI

c.

EcorI

d.

EcoRI

Discuss
Answer: (d).EcoRI
Q47.
Why is DNase preferred over restriction endonuclease in some cases?

a.

DNase is preferred over restriction endonuclease in some cases because the latter are not able to recognize some of the restriction sites

b.

DNase is more specific as restriction endonuclease, so the required fragment is obtained

c.

DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments

d.

DNase is abundant in comparison the restriction endonuclease

Discuss
Answer: (c).DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments
Q48.
The ends created by use of DNase have unique single stranded sequences.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q49.
Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true?

a.

Sonication, needles, syringes, etc all come under the category of physical stress

b.

In physical stress, there are no chances of contamination

c.

The ends obtained have unique sequences

d.

They are less effective than DNase treatment

Discuss
Answer: (a).Sonication, needles, syringes, etc all come under the category of physical stress
Q50.
Phosphatases refer to __________

a.

the enzymes which add phosphate group at the end of the DNA molecule in the place of hydroxyl group

b.

the enzymes which hydrolytically remove phosphate group from the DNA molecules and replace them with hydroxyl group

c.

the enzymes responsible for removal of phosphate group from the DNA molecules and replace them with hydrogen

d.

the enzymes responsible for replacing hydrogen in the DNA molecules with the phosphate group

Discuss
Answer: (b).the enzymes which hydrolytically remove phosphate group from the DNA molecules and replace them with hydroxyl group
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