Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 5 of 24

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Q41.
The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:

a.

star activity

b.

specificity elevation

c.

concentration gradient effects

d.

diamond activity

Discuss
Answer: (a).star activity
Q42.
Which of the following statements is correct with respect to a unit of an enzyme?

a.

One unit of enzyme is defined as the amount of enzyme required to digest 1miligram of standard DNA in a specific time of 1hr and under given temperature conditions

b.

The amount of enzyme required doesn’t vary with the number of sites present in the DNA

c.

If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites

d.

The amount of enzyme required for digestion of DNA with less number of sites is more than that of more number of sites in the same amount of DNA

Discuss
Answer: (c).If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites
Q43.
Which of the following statement is correct regarding partial digestion?

a.

It is defined as the conditions where all the sites in the DNA sequence are not recognized

b.

The number of fragments created by partial digestion are same as that of complete digestion

c.

It is not useful in representation of genomic library

d.

Exactly half of the sites in the DNA are recognized

Discuss
Answer: (a).It is defined as the conditions where all the sites in the DNA sequence are not recognized
Q44.
Which of the following is the correct nomenclature of a restriction enzyme obtained from the first activity of strain R of Escherichia coli?

a.

EcoR1

b.

EscRI

c.

EcorI

d.

EcoRI

Discuss
Answer: (d).EcoRI
Q45.
Why is DNase preferred over restriction endonuclease in some cases?

a.

DNase is preferred over restriction endonuclease in some cases because the latter are not able to recognize some of the restriction sites

b.

DNase is more specific as restriction endonuclease, so the required fragment is obtained

c.

DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments

d.

DNase is abundant in comparison the restriction endonuclease

Discuss
Answer: (c).DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments
Q46.
The ends created by use of DNase have unique single stranded sequences.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q47.
Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true?

a.

Sonication, needles, syringes, etc all come under the category of physical stress

b.

In physical stress, there are no chances of contamination

c.

The ends obtained have unique sequences

d.

They are less effective than DNase treatment

Discuss
Answer: (a).Sonication, needles, syringes, etc all come under the category of physical stress
Q48.
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

a.

Wedge gels

b.

Buffer-gradient gels

c.

Pulse field gels

d.

Varying the amount of agarose will carry out the separation

Discuss
Answer: (c).Pulse field gels
Q49.
Which of the following statement is correct for the method of separation of large molecules?

a.

The large DNA molecules can pass through the matrix in any direction, not necessarily in the direction parallel to the movement

b.

The separation can be carried out if the fields are at right angles to each other

c.

The time of pulse should be less than that of the reorientation time

d.

Separation is not of the megabase level

Discuss
Answer: (b).The separation can be carried out if the fields are at right angles to each other
Q50.
The random change in allele frequencies is

a.

genetic drift

b.

diversity

c.

linkage

d.

Gene pool

Discuss
Answer: (a).genetic drift
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