Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 3 of 24

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Q21.
Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?

a.

Circular

b.

Supercoiled

c.

Nicked

d.

Nicked

Discuss
Answer: (b).Supercoiled
Q22.
How is the size of molecules under analysis measured?

a.

By measuring the distance moved through a ruler

b.

By measuring the amount of visualising dye used

c.

By running a standard molecule, whose size is known in parallel

d.

There is no exact criterion for doing so

Discuss
Answer: (c).By running a standard molecule, whose size is known in parallel
Q23.
After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

a.

The ends created are always single stranded

b.

The ends created are always double stranded

c.

Either the ends are single stranded or they are double stranded

d.

One end is single stranded and one end is double stranded

Discuss
Answer: (c).Either the ends are single stranded or they are double stranded
Q24.
A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends?

a.

The 5’ end is having hydroxyl group

b.

The 5’ end is having phosphate group

c.

The 3’ end is having phosphate group

d.

Any group can be present at any end

Discuss
Answer: (b).The 5’ end is having phosphate group
Q25.
Blunt ends created by the restriction endonuclease can be joined.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q26.
The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it?

a.

The ends created are double stranded

b.

The single stranded end is 5’ in nature

c.

The single stranded end is 3’ in nature

d.

To decide about the nature of the ends more information is needed

Discuss
Answer: (b).The single stranded end is 5’ in nature
Q27.
The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?

a.

The ends created by both the enzymes are compatible

b.

The ends created by both the enzymes are not-compatible

c.

The ends created by DpnI are single stranded

d.

The ends created by Sau3A are single stranded

Discuss
Answer: (b).The ends created by both the enzymes are not-compatible
Q28.
The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it?

a.

The molecules which are cut by the same enzyme, anneal only if the sequence is palindromic in nature

b.

When the molecules are cleaved by the same enzyme and the recognition sequence is palindromic in nature, there is no effect on annealing

c.

There are increased chances of annealing if the recognition sequence is palindromic in nature

d.

The term ‘palindromic’ can be used whether the sequence is read from 5’ to 3’ or 3’ to 5’

Discuss
Answer: (c).There are increased chances of annealing if the recognition sequence is palindromic in nature
Q29.
If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif?

a.

1/256

b.

1/64

c.

1/16

d.

1/8

Discuss
Answer: (a).1/256
Q30.
Isoschizomers are defined as ___________

a.

enzymes having same recognition sequence and always cutting at the same site

b.

enzymes having same recognition sequence and always cutting at different site

c.

enzymes having different recognition site and cutting at the same site

d.

enzymes having same recognition site and they may or may not cut at the same site

Discuss
Answer: (d).enzymes having same recognition site and they may or may not cut at the same site
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