Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 3 of 24

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Q21.
Which of the following statement is correct for the method of separation of large molecules?

a.

The large DNA molecules can pass through the matrix in any direction, not necessarily in the direction parallel to the movement

b.

The separation can be carried out if the fields are at right angles to each other

c.

The time of pulse should be less than that of the reorientation time

d.

Separation is not of the megabase level

Discuss
Answer: (b).The separation can be carried out if the fields are at right angles to each other
Q22.
How many approaches are there in order to clone the complete genome?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q23.
If a full proteomic analysis of growth medium is carried out and is combined with ____________ genome sequence, genes for other _____________ proteins are also obtained.

a.

partial, defensive

b.

partial, secreted

c.

complete, defensive

d.

complete, secreted

Discuss
Answer: (d).complete, secreted
Q24.
If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?

a.

The protein is not protease

b.

The protein can be incorrectly folded which can block the protease activity

c.

There might be some other cofactor required for protease activity

d.

The most commonly used expression system is E.coli

Discuss
Answer: (a).The protein is not protease
Q25.
For getting a large amount of proteins to crystallize, which of the following should be used as an expression system?

a.

Bacterial system

b.

Yeast systems

c.

Eukaryotic systems

d.

Both eukaryotic and bacterial systems can be used

Discuss
Answer: (d).Both eukaryotic and bacterial systems can be used
Q26.
If a mutation perturbs the structure, then stability and folding are not affected.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (b).False
Q27.
The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________

a.

reflects, can be determined

b.

reflects, can’t be determined

c.

doesn’t necessarily reflects, can be determined

d.

doesn’t necessarily reflects, can’t be determined

Discuss
Answer: (d).doesn’t necessarily reflects, can’t be determined
Q28.
For a convenient transformation system, _____ can be used for gene silencing.

a.

antisense RNA

b.

transposon insertion

c.

either antisense RNA or transposon insertion

d.

transposon insertion followed by antisense RNA

Discuss
Answer: (a).antisense RNA
Q29.
The term ‘endonuclease’ refers to cutting the DNA sequence from ______________

a.

only within the polynucleotide chain, not at the ends

b.

the ends of the chain

c.

anywhere in the chain

d.

exactly in the middle of the chain

Discuss
Answer: (a).only within the polynucleotide chain, not at the ends
Q30.
The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA?

a.

By methylation of bacterial DNA by restriction enzyme

b.

By methylation of foreign DNA by restriction enzyme

c.

By phosphorylation of bacterial DNA by restriction enzyme

d.

By phosphorylation of foreign DNA by restriction enzyme

Discuss
Answer: (a).By methylation of bacterial DNA by restriction enzyme
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