Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 3 of 24

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Q21.
The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it?

a.

The ends created are double stranded

b.

The single stranded end is 5’ in nature

c.

The single stranded end is 3’ in nature

d.

To decide about the nature of the ends more information is needed

Discuss
Answer: (b).The single stranded end is 5’ in nature
Q22.
Which of the following is correct with respect to caesium chloride centrifugation?

a.

Caesium is light in weight

b.

The dissolution of caesium and nucleic acids leads to the formation of gradients

c.

According to the amount of supercoiling and A+T content, the DNA settles

d.

Nicked DNA settles below than supercoiled DNA

Discuss
Answer: (b).The dissolution of caesium and nucleic acids leads to the formation of gradients
Q23.
Which of the following components settles at the bottom?

a.

RNA

b.

Proteins

c.

Nicked DNA

d.

Supercoiled DNA

Discuss
Answer: (a).RNA
Q24.
The location of plasmid DNA can be visualized by addition of:

a.

bromophenol blue

b.

ethidium bromide

c.

ortho xylene

d.

texas red

Discuss
Answer: (b).ethidium bromide
Q25.
Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?

a.

Circular

b.

Supercoiled

c.

Nicked

d.

Nicked

Discuss
Answer: (b).Supercoiled
Q26.
How is the size of molecules under analysis measured?

a.

By measuring the distance moved through a ruler

b.

By measuring the amount of visualising dye used

c.

By running a standard molecule, whose size is known in parallel

d.

There is no exact criterion for doing so

Discuss
Answer: (c).By running a standard molecule, whose size is known in parallel
Q27.
Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?

a.

Agarose is a polysaccharide which is obtained from red algae

b.

Agarose is a polysaccharide obtained from fungus

c.

It is composed of glucose residues

d.

It is obtained via dissolving in its water by boiling in water and then cooling it

Discuss
Answer: (a).Agarose is a polysaccharide which is obtained from red algae
Q28.
Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

a.

They are obtained via polymerization between acrylamide and bis-acrylamide

b.

The components added for initiating polymerization are ammonium persulphate and TEMED

c.

It is casted in horizontal and flat trays

d.

TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

Discuss
Answer: (c).It is casted in horizontal and flat trays
Q29.
If the amount of agarose added is more, the molecular under analysis should have the following characteristic:

a.

small size

b.

large size

c.

size has no relation with the amount of agarose

d.

the amount of molecules under analysis matters

Discuss
Answer: (a).small size
Q30.
In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?

a.

Renaturing

b.

Denaturing

c.

The routine agarose gel

d.

The routine polyacrylamide gel

Discuss
Answer: (b).Denaturing
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