Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 3 of 24

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Q21.
The term ‘endonuclease’ refers to cutting the DNA sequence from ______________

a.

only within the polynucleotide chain, not at the ends

b.

the ends of the chain

c.

anywhere in the chain

d.

exactly in the middle of the chain

Discuss
Answer: (a).only within the polynucleotide chain, not at the ends
Q22.
The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA?

a.

By methylation of bacterial DNA by restriction enzyme

b.

By methylation of foreign DNA by restriction enzyme

c.

By phosphorylation of bacterial DNA by restriction enzyme

d.

By phosphorylation of foreign DNA by restriction enzyme

Discuss
Answer: (a).By methylation of bacterial DNA by restriction enzyme
Q23.
Even after replication, how the modified DNA remains protected?

a.

It remains protected because of conservative mode of replication

b.

It remains protected because of semi-conservative mode of replication

c.

The mode of replication has no role to play in the protection

d.

It is again modified after replication

Discuss
Answer: (b).It remains protected because of semi-conservative mode of replication
Q24.
How many classes of restriction enzymes are there?

a.

2

b.

1

c.

3

d.

4

Discuss
Answer: (c).3
Q25.
Type II cuts the sequence in the following way __________

a.

Within the recognition sequence

b.

At 100-1000 nucleotides away from the recognition sequence

c.

At 27-30 nucleotides away from the recognition sequence

d.

It cuts randomly

Discuss
Answer: (a).Within the recognition sequence
Q26.
After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

a.

The ends created are always single stranded

b.

The ends created are always double stranded

c.

Either the ends are single stranded or they are double stranded

d.

One end is single stranded and one end is double stranded

Discuss
Answer: (c).Either the ends are single stranded or they are double stranded
Q27.
A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends?

a.

The 5’ end is having hydroxyl group

b.

The 5’ end is having phosphate group

c.

The 3’ end is having phosphate group

d.

Any group can be present at any end

Discuss
Answer: (b).The 5’ end is having phosphate group
Q28.
Blunt ends created by the restriction endonuclease can be joined.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q29.
The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it?

a.

The ends created are double stranded

b.

The single stranded end is 5’ in nature

c.

The single stranded end is 3’ in nature

d.

To decide about the nature of the ends more information is needed

Discuss
Answer: (b).The single stranded end is 5’ in nature
Q30.
The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?

a.

The ends created by both the enzymes are compatible

b.

The ends created by both the enzymes are not-compatible

c.

The ends created by DpnI are single stranded

d.

The ends created by Sau3A are single stranded

Discuss
Answer: (b).The ends created by both the enzymes are not-compatible
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