Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 7 of 24

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Q61.
Which of the following statements is correct with respect to exonuclease?

a.

They only act on single stranded DNA molecules

b.

They only act on double stranded DNA molecules

c.

They remove a single nucleotide base at a time

d.

They remove nucleotide bases from the middle of a polynucleotide chain

Discuss
Answer: (c).They remove a single nucleotide base at a time
Q62.
How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q63.
What is the mode of action of exonuclease III?

a.

Exonuclease III acts on single stranded DNA in 3’-5’ direction

b.

Exonuclease III acts on double stranded DNA in 5’-3’ direction

c.

Exonuclease III acts on single stranded DNA in 5’-3’ direction

d.

Exonuclease III acts on double stranded DNA in 3’-5’ direction

Discuss
Answer: (d).Exonuclease III acts on double stranded DNA in 3’-5’ direction
Q64.
Which of the following statements is correct regarding S1 nuclease?

a.

It acts on double stranded DNA

b.

It acts on single stranded DNA

c.

It acts on both types of strands

d.

It is obtained from E. coli

Discuss
Answer: (b).It acts on single stranded DNA
Q65.
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

a.

Wedge gels

b.

Buffer-gradient gels

c.

Pulse field gels

d.

Varying the amount of agarose will carry out the separation

Discuss
Answer: (c).Pulse field gels
Q66.
Which of the following statement is correct for the method of separation of large molecules?

a.

The large DNA molecules can pass through the matrix in any direction, not necessarily in the direction parallel to the movement

b.

The separation can be carried out if the fields are at right angles to each other

c.

The time of pulse should be less than that of the reorientation time

d.

Separation is not of the megabase level

Discuss
Answer: (b).The separation can be carried out if the fields are at right angles to each other
Q67.
The random change in allele frequencies is

a.

genetic drift

b.

diversity

c.

linkage

d.

Gene pool

Discuss
Answer: (a).genetic drift
Q68.
The effect of genetic drift increases as the population size

a.

increases

b.

decreases

c.

remains same

d.

Grown vigorously

Discuss
Answer: (b).decreases
Q69.
What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?

a.

The molecule is shortened only from 3’ end

b.

The molecule is shortened only from 5’ end

c.

The molecule is shortened from both the ends

d.

Only Exonuclease acts and S1 doesn’t acts

Discuss
Answer: (c).The molecule is shortened from both the ends
Q70.
How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

a.

By using Phosphorothioate nucleotide analogue

b.

By making both the ends double stranded in nature

c.

By labelling one end with a radioactive compound

d.

By increasing the time of exposure of the DNA molecule to the enzyme

Discuss
Answer: (a).By using Phosphorothioate nucleotide analogue
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