Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 7 of 24

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Q61.
Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

a.

RNA dependent DNA polymerase

b.

DNA dependent RNA polymerase

c.

RNA dependent RNA polymerase

d.

DNA dependent DNA polymerase

Discuss
Answer: (b).DNA dependent RNA polymerase
Q62.
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?

a.

They only add a single nucleotide

b.

They only add a string of nucleotides and not a single nucleotide

c.

Terminal transferase adds a series of nucleotides at the 3’ end

d.

Taq polymerase adds a single nucleotide at the 5’ end of the PCR product

Discuss
Answer: (c).Terminal transferase adds a series of nucleotides at the 3’ end
Q63.
Which of the following statements is correct with respect to exonuclease?

a.

They only act on single stranded DNA molecules

b.

They only act on double stranded DNA molecules

c.

They remove a single nucleotide base at a time

d.

They remove nucleotide bases from the middle of a polynucleotide chain

Discuss
Answer: (c).They remove a single nucleotide base at a time
Q64.
How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q65.
What is the mode of action of exonuclease III?

a.

Exonuclease III acts on single stranded DNA in 3’-5’ direction

b.

Exonuclease III acts on double stranded DNA in 5’-3’ direction

c.

Exonuclease III acts on single stranded DNA in 5’-3’ direction

d.

Exonuclease III acts on double stranded DNA in 3’-5’ direction

Discuss
Answer: (d).Exonuclease III acts on double stranded DNA in 3’-5’ direction
Q66.
Which of the following statements is correct regarding S1 nuclease?

a.

It acts on double stranded DNA

b.

It acts on single stranded DNA

c.

It acts on both types of strands

d.

It is obtained from E. coli

Discuss
Answer: (b).It acts on single stranded DNA
Q67.
What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?

a.

The molecule is shortened only from 3’ end

b.

The molecule is shortened only from 5’ end

c.

The molecule is shortened from both the ends

d.

Only Exonuclease acts and S1 doesn’t acts

Discuss
Answer: (c).The molecule is shortened from both the ends
Q68.
How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

a.

By using Phosphorothioate nucleotide analogue

b.

By making both the ends double stranded in nature

c.

By labelling one end with a radioactive compound

d.

By increasing the time of exposure of the DNA molecule to the enzyme

Discuss
Answer: (a).By using Phosphorothioate nucleotide analogue
Q69.
Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?

a.

It is having only 3’-5’ exonuclease activity and no endonuclease activity

b.

It is having only an endonuclease activity

c.

It is having 5’-3’ exonuclease activity

d.

It leads to shortening from both the ends

Discuss
Answer: (d).It leads to shortening from both the ends
Q70.
The extent of deletions can be manipulated by controlling which of them?

a.

Time of incubation only

b.

Amount of nuclease added

c.

They both have a role to play in the extent of the deletion

d.

The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time

Discuss
Answer: (c).They both have a role to play in the extent of the deletion
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