Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 7 of 24

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Q61.
Which of the following statements is correct regarding S1 nuclease?

a.

It acts on double stranded DNA

b.

It acts on single stranded DNA

c.

It acts on both types of strands

d.

It is obtained from E. coli

Discuss
Answer: (b).It acts on single stranded DNA
Q62.
What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?

a.

The molecule is shortened only from 3’ end

b.

The molecule is shortened only from 5’ end

c.

The molecule is shortened from both the ends

d.

Only Exonuclease acts and S1 doesn’t acts

Discuss
Answer: (c).The molecule is shortened from both the ends
Q63.
How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

a.

By using Phosphorothioate nucleotide analogue

b.

By making both the ends double stranded in nature

c.

By labelling one end with a radioactive compound

d.

By increasing the time of exposure of the DNA molecule to the enzyme

Discuss
Answer: (a).By using Phosphorothioate nucleotide analogue
Q64.
Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?

a.

It is having only 3’-5’ exonuclease activity and no endonuclease activity

b.

It is having only an endonuclease activity

c.

It is having 5’-3’ exonuclease activity

d.

It leads to shortening from both the ends

Discuss
Answer: (d).It leads to shortening from both the ends
Q65.
The extent of deletions can be manipulated by controlling which of them?

a.

Time of incubation only

b.

Amount of nuclease added

c.

They both have a role to play in the extent of the deletion

d.

The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time

Discuss
Answer: (c).They both have a role to play in the extent of the deletion
Q66.
What is the function of methylase?

a.

Addition of methyl groups to DNA

b.

Removal of methyl groups from DNA

c.

Both in removal and addition of methyl groups from DNA

d.

It is used in production of methane gas

Discuss
Answer: (a).Addition of methyl groups to DNA
Q67.
Methylase is useful in cloning experiments.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q68.
Ligation is defined as ______________

a.

Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands

b.

Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands

c.

Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands

d.

Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

Discuss
Answer: (c).Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands
Q69.
If only one bond is broken in the sugar-phosphate backbone, it is called as ___________

a.

gap

b.

nick

c.

break

d.

leakage

Discuss
Answer: (b).nick
Q70.
How many categories of ligation reaction are there on the basis of ends created?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
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