DNA and Genomic Sequencing MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on DNA and Genomic Sequencing, a fundamental topic in the field of Bioinformatics. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our DNA and Genomic Sequencing MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of DNA and Genomic Sequencing mcq questions that explore various aspects of DNA and Genomic Sequencing problems. Each MCQ is crafted to challenge your understanding of DNA and Genomic Sequencing principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Bioinformatics tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our DNA and Genomic Sequencing MCQs are your pathway to success in mastering this essential Bioinformatics topic.

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DNA and Genomic Sequencing MCQs | Page 13 of 17

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Q121.
Which of the given statements is incorrect about All-against-all Self-comparison?

a.

A comparison of each protein in the proteome with all other proteins distinguishes unique proteins from proteins that have arisen from gene duplication, and also reveals the number of protein families but the domain content of these proteins cannot be known

b.

In all-against-all proteome comparison, each protein is used as a query in a similarity search against the remaining proteome

c.

In all-against-all proteome comparison, the similar sequences are ranked by the quality and length of the alignments found

d.

In all-against-all proteome comparison, The search is conducted with each alignment score receiving a statistical evaluation (P or E value)

Discuss
Answer: (a).A comparison of each protein in the proteome with all other proteins distinguishes unique proteins from proteins that have arisen from gene duplication, and also reveals the number of protein families but the domain content of these proteins cannot be known
Q122.
Which of the given statements is incorrect about Cluster analysis?

a.

Clustering organizes the proteins into groups by some objective criterion

b.

One criterion for a matching protein pair is the statistical significance of their alignment score

c.

The P or E value from BLAST searches cannot be the criterion for a matching protein pair

d.

A criterion for clustering proteins is the distance between each pair of sequences in a multiple sequence alignment

Discuss
Answer: (c).The P or E value from BLAST searches cannot be the criterion for a matching protein pair
Q123.
Which of the given statements is incorrect about Clustering by making subgraphs?

a.

Each sequence is a vertex and each pair of sequences that is matched with a significant alignment score is joined by an edge that is weighted according to the statistical significance of the alignment score

b.

One way to identify the most strongly supported clusters is simply to add the most weakly supported edges in the graph

c.

One way to identify the most strongly supported clusters is simply to remove the most weakly supported edges in the graph

d.

An edge is weighted according to the statistical significance of the alignment score

Discuss
Answer: (c).One way to identify the most strongly supported clusters is simply to remove the most weakly supported edges in the graph
Q124.
Which of the given statements is incorrect about Clustering by single linkage?

a.

In First step, a group of related sequences found in the all-against-all proteome comparison is subjected to a multiple sequence alignment usually by CLUSTALW

b.

A neighbor-joining algorithm is rarely used in this method

c.

This procedure and the algorithms are the same as those used to make a phylogenetic tree by the distance methods

d.

A distance matrix that shows the number of amino acid changes between each pair of sequences is made

Discuss
Answer: (b).A neighbor-joining algorithm is rarely used in this method
Q125.
The all-against-all analyses provide an indication as to the number of protein/gene families in an organism. This number represents the core proteome of the organism from which all biological functions have diversified.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q126.
Which of the given statements is incorrect about Grouping Sequences?

a.

The problem of deciding which sequences to include in the same group or cluster and which to separate into different groups or clusters is a recurring one

b.

Divergence is necessary, but the sequences chosen should be clearly related based on inspection of each pair-wise alignment and a statistical analysis

c.

The conservative approach is to group distinct sequences

d.

The adventurous approach is to choose a set of marginally alignable sequences to pursue the difficult task of making a multiple sequence alignment and then to make profile models that may recognize divergence but will also give false predictions

Discuss
Answer: (c).The conservative approach is to group distinct sequences
Q127.
Which of the given statements is incorrect about Clusters of orthologous groups?

a.

Using the protein from one of the organisms to search the proteome of the other for high-scoring matches should identify the ortholog as the highest- scoring match, or best hit

b.

When entire proteomes of the two organisms are available, orthologs may be identified

c.

a pair of orthologous genes in two organisms share so much sequence similarity that they may be assumed to have arisen from a common ancestor gene

d.

each of the orthologs belongs to a family composed of paralogous sequences but irrelevant or not related to each other

Discuss
Answer: (d).each of the orthologs belongs to a family composed of paralogous sequences but irrelevant or not related to each other
Q128.
Which of the given statements is incorrect about Clusters of orthologous groups?

a.

Paralogs may include a best hit or a high-scoring match of one of the sequences by another, but the reciprocal match can have low similarity that does not have to be significant

b.

Paralogs defined by sets of three matching sequences in the selected organisms were kept separated from the clusters

c.

Orthologous pairs were first defined by the best hits in reciprocal searches

d.

To produce COGs, similarity searches were performed among the proteomes of phylogenetically distinct clades of prokaryotes

Discuss
Answer: (b).Paralogs defined by sets of three matching sequences in the selected organisms were kept separated from the clusters
Q129.
Which of the given statements is incorrect about the Comparison of proteomes to EST databases of an organism?

a.

ESTs are single DNA sequence reads that contain a small fraction of incorrect base assessments, insertions, and deletions

b.

Many sequences arise from near the 5’ end of the mRNA, although every effort is usually made to read as far 3’ as possible into the upstream portion of the cDNA

c.

EST libraries are useful for preliminary identification of genes by database similarity searches

d.

An EST database of an organism can be analyzed for the presence of gene families,
orthologs, and paralogs

Discuss
Answer: (b).Many sequences arise from near the 5’ end of the mRNA, although every effort is usually made to read as far 3’ as possible into the upstream portion of the cDNA
Q130.
Which of the given statements is incorrect about Searching for orthologs to a protein family in an EST database?

a.

Searches of EST databases for matches to a query sequence routinely produce minimal amounts of output that must be searched manually for significant hits

b.

ESTs with a high percent identity with the query sequence, a long alignment with the query sequence, and a very low E value of the alignment score represent groups of paralogous and orthologous genes

c.

To identify orthologs as the most closely related sequence, ESTs were aligned using the amino acid alignment as a guide

d.

To identify orthologs as the most closely related sequence, a phylogenetic tree was produced by the maximum likelihood method

Discuss
Answer: (a).Searches of EST databases for matches to a query sequence routinely produce minimal amounts of output that must be searched manually for significant hits
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