Modifications and Mutagenesis MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Modifications and Mutagenesis, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Modifications and Mutagenesis MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Modifications and Mutagenesis mcq questions that explore various aspects of Modifications and Mutagenesis problems. Each MCQ is crafted to challenge your understanding of Modifications and Mutagenesis principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Modifications and Mutagenesis MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Modifications and Mutagenesis. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Modifications and Mutagenesis knowledge to the test? Let's get started with our carefully curated MCQs!

Modifications and Mutagenesis MCQs | Page 3 of 8

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Q21.
A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

a.

two, two

b.

two, three

c.

one, two

d.

one, three

Discuss
Answer: (b).two, three
Q22.
Choose the correct statement.

a.

There are two flanking primers and they are having mutations

b.

There is one primer which anneals the target sequence and is having mutation

c.

Either of the flanking primers or the primer is annealing to the target sequence is having mutation

d.

One of the flanking primer and the primer annealing to the target sequence is having mutation

Discuss
Answer: (b).There is one primer which anneals the target sequence and is having mutation
Q23.
PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q24.
Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

a.

Double stranded circular molecule is used as a template

b.

Mutation is introduced into one of flanking primers

c.

Single stranded circular molecule is used as a template

d.

After amplification, mutation is introduced into one strand

Discuss
Answer: (a).Double stranded circular molecule is used as a template
Q25.
If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

a.

mutagenic PCR

b.

error-prone PCR

c.

random PCR

d.

general PCR

Discuss
Answer: (b).error-prone PCR
Q26.
For the selection of the molecules having mutated sequence, which of the statement is true?

a.

It is suitable for methods which are PCR based

b.

It is suitable for methods which are not PCR based

c.

It is suitable for both PCR and not PCR based

d.

Selection of molecules with the mutant sequence is not possible

Discuss
Answer: (b).It is suitable for methods which are not PCR based
Q27.
Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

a.

A vector is used which is having antibiotic resistance gene

b.

Apart from antibiotic resistance gene, a second antibiotic resistance gene is also present

c.

There are two mutagenic primers which are used

d.

The second strand synthesis is carried out by only one primer

Discuss
Answer: (d).The second strand synthesis is carried out by only one primer
Q28.
Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

a.

mutS mutation

b.

mutD mutation

c.

mutE mutation

d.

mutG mutation

Discuss
Answer: (a).mutS mutation
Q29.
Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q30.
In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

a.

Magnesium

b.

Calcium

c.

Sodium

d.

Sulphur

Discuss
Answer: (d).Sulphur
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