Modifications and Mutagenesis MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Modifications and Mutagenesis, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Modifications and Mutagenesis MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Modifications and Mutagenesis mcq questions that explore various aspects of Modifications and Mutagenesis problems. Each MCQ is crafted to challenge your understanding of Modifications and Mutagenesis principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Modifications and Mutagenesis MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Modifications and Mutagenesis. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Modifications and Mutagenesis knowledge to the test? Let's get started with our carefully curated MCQs!

Modifications and Mutagenesis MCQs | Page 2 of 8

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Q11.
Bisulphite ions are used to deaminate ________ residues in _______ DNA.

a.

C, double stranded

b.

C, single stranded

c.

U, double stranded

d.

U, single stranded

Discuss
Answer: (b).C, single stranded
Q12.
For mutagenesis without PCR, which of the following can be used as a template?

a.

Single stranded DNA

b.

Double stranded DNA

c.

Circular DNA

d.

Both single and double stranded DNA

Discuss
Answer: (d).Both single and double stranded DNA
Q13.
An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

a.

complementary

b.

non-complementary

c.

can either be complementary or non-complementary

d.

not related

Discuss
Answer: (a).complementary
Q14.
If the template is double stranded, they need to be separated before annealing of oligonucleotide.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q15.
Which of the following statement is incorrect for the synthesis of the second strand?

a.

The oligonucleotide is acting as a primer for the synthesis of the second strand

b.

DNA polymerase and dNTPs are added for synthesis

c.

The polymerase should have 5’-3’ exonuclease activity

d.

A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence

Discuss
Answer: (c).The polymerase should have 5’-3’ exonuclease activity
Q16.
Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q17.
How many sites can be mutated at a time?

a.

1

b.

2

c.

3

d.

Many

Discuss
Answer: (d).Many
Q18.
Several different mutations can be induced at one site.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q19.
If mixed oligonucleotides are used, it is regarded as ______________

a.

mixed mutagenesis

b.

multiple mutagenesis

c.

cassette mutagenesis

d.

polymutagenesis

Discuss
Answer: (c).cassette mutagenesis
Q20.
It is easier to subclone a restriction fragment if it belongs to?

a.

small gene

b.

large gene

c.

prokaryotic organism

d.

eukaryotic organism

Discuss
Answer: (b).large gene
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