Use of Cloned DNA MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Use of Cloned DNA, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Use of Cloned DNA MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Use of Cloned DNA mcq questions that explore various aspects of Use of Cloned DNA problems. Each MCQ is crafted to challenge your understanding of Use of Cloned DNA principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Use of Cloned DNA MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Use of Cloned DNA. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

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Use of Cloned DNA MCQs | Page 2 of 8

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Q11.
Isolation of RNA can be carried out easily from bacterial cells.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q12.
Promoters are generally used after isolation from ___________

a.

bacteriophage T7

b.

bacteriophage SP6

c.

baceriophage Mu

d.

both bacteriophage T7 and SP6

Discuss
Answer: (d).both bacteriophage T7 and SP6
Q13.
By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______

a.

transcription, regardless of orientation

b.

transcription, only in one orientation

c.

translation, regardless of orientation

d.

translation, only in one orientation

Discuss
Answer: (a).transcription, regardless of orientation
Q14.
Guanylyl transferase and GTP are used for?

a.

Transcription

b.

Translation

c.

Capping of the message

d.

Packaging

Discuss
Answer: (c).Capping of the message
Q15.
Capping can be introduced by the use of cap analogue. Which of the statement is true?

a.

Cap analogue can be introduced at the end of the transcript

b.

Cap analogue can be introduced at the start of the transcript

c.

Cap analogue can be introduced anywhere in the transcript

d.

Cap analogue can be introduced both at the end and starting of the transcript

Discuss
Answer: (b).Cap analogue can be introduced at the start of the transcript
Q16.
If transcription should not be carried out beyond the insert in the vector, then it should be linearized.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q17.
If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium?

a.

Single stranded DNA

b.

Double stranded DNA

c.

Both single and double stranded DNA

d.

Circular DNA

Discuss
Answer: (c).Both single and double stranded DNA
Q18.
Little quantities of radiolabelled proteins are required for which of the following?

a.

co or post translational targeting

b.

modification of proteins

c.

both co or post translational targeting and modification of proteins

d.

crystallization for structural studies

Discuss
Answer: (c).both co or post translational targeting and modification of proteins
Q19.
__________ quantities of _______ protein are required for determination of properties in biochemical and biological assays.

a.

Small, non-radiolabelled

b.

Small, radiolabelled

c.

Large, radiolablelled

d.

Large, non-radiolabelled

Discuss
Answer: (d).Large, non-radiolabelled
Q20.
Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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