Use of Cloned DNA MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Use of Cloned DNA, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Use of Cloned DNA MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Use of Cloned DNA mcq questions that explore various aspects of Use of Cloned DNA problems. Each MCQ is crafted to challenge your understanding of Use of Cloned DNA principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Use of Cloned DNA MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Use of Cloned DNA MCQs | Page 3 of 8

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Q21.
For the production of unlabelled and huge amount of proteins, which of the following is true?

a.

Transcription is carried out in vivo and translation in vitro

b.

Transcription and translation both are carried out in vivo

c.

Transcription and translation both are carried out in vitro

d.

Transcription is carried out in vitro and translation in vivo

Discuss
Answer: (b).Transcription and translation both are carried out in vivo
Q22.
How many methods are there, which are used for protein synthesis in vitro?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (c).3
Q23.
Reticulocytes are immature red cells, which are obtained from ___________

a.

rabbit

b.

pig

c.

human

d.

cow

Discuss
Answer: (a).rabbit
Q24.
What is the use of adding micrococcal nuclease in the reticulocyte cells?

a.

It degrades DNA

b.

It degrades mRNA

c.

It degrades proteins

d.

It degrades RNA and untranslated DNA

Discuss
Answer: (b).It degrades mRNA
Q25.
Treatment of reticulocyte cells is done with EGTA. It chelates the calcium ions which are required for functioning of micrococcal nuclease.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q26.
In ________ transcription and translation is coupled.

a.

prokaryotes

b.

eukaryotes

c.

both prokaryotes and eukaryotes

d.

both prokaryotes and eukaryotes, but favourable in eukaryotes

Discuss
Answer: (a).prokaryotes
Q27.
In which of the following systems, transcription and translation are carried out together?

a.

Reticuloycte lysate

b.

Wheat gram extract

c.

Both in reticulocyte lysate and wheat gram extract

d.

S-30 extract

Discuss
Answer: (d).S-30 extract
Q28.
If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

a.

few, translation

b.

few, transformation

c.

no, transformation

d.

no, translation

Discuss
Answer: (c).no, transformation
Q29.
Ribosome binding site in E.coli is composed of which sequence primarily?

a.

-GAGG-

b.

-CTCC-

c.

-ATGG-

d.

-CGTT-

Discuss
Answer: (a).-GAGG-
Q30.
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

a.

ACG

b.

AUG

c.

UAC

d.

CAG

Discuss
Answer: (b).AUG
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