Use of Cloned DNA MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Use of Cloned DNA, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Use of Cloned DNA MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Use of Cloned DNA mcq questions that explore various aspects of Use of Cloned DNA problems. Each MCQ is crafted to challenge your understanding of Use of Cloned DNA principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Use of Cloned DNA MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Use of Cloned DNA MCQs | Page 5 of 8

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Q41.
Choose the correct statement for rifampcin.

a.

It inactivates both E. coli polymerase and T7 polymerase

b.

It activates both E. coli polymerase and T7 polymerase

c.

It inhibits T7 polymerase but doesn’t inhibits E. coli polymerase

d.

It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase

Discuss
Answer: (d).It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase
Q42.
The ______ operon encodes proteins involved in arabinose metabolism.

a.

araBCD

b.

araABD

c.

araBAD

d.

araDBA

Discuss
Answer: (c).araBAD
Q43.
tac promoter is an example of which type of promoter?

a.

hybrid promoter

b.

fusion promoter

c.

lacZ promoter

d.

araBAD promoter

Discuss
Answer: (a).hybrid promoter
Q44.
The tac promoter is made by _____ region of trp promoter and _____ region of the lacUV5 promoter.

a.

10, 35

b.

35, 10

c.

10, 10

d.

35, 35

Discuss
Answer: (b).35, 10
Q45.
The tac promoter includes the lac operator and is regulated by a repressor.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q46.
Expression of T7 promoter- lac operator hybrid requires ___________

a.

T7 RNA polymerase

b.

An inducer such as IPTG

c.

Both T7 RNA polymerase and inducer such as IPTG

d.

T7 DNA polymerase

Discuss
Answer: (c).Both T7 RNA polymerase and inducer such as IPTG
Q47.
When lacUV5 control system is used, addition of IPTG _______ the expression of _________

a.

activates, T7 RNA polymerase

b.

inactivates, T7 RNA polymerase

c.

activates, T7 DNA polymerase

d.

inactivates, T7 DNA polymerase

Discuss
Answer: (a).activates, T7 RNA polymerase
Q48.
There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation.

a.

solubility, phosphodiester bond formation

b.

insolubility, phosphodiester bond formation

c.

solubility, disulphide bond formation

d.

insolubility, disulphidebond formation

Discuss
Answer: (c).solubility, disulphide bond formation
Q49.
A short peptide region fused to a protein of interest is known as ___________

a.

tag

b.

oligonucleotide

c.

fragment

d.

dimer

Discuss
Answer: (a).tag
Q50.
Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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