Use of Cloned DNA MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Use of Cloned DNA, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Use of Cloned DNA MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Use of Cloned DNA mcq questions that explore various aspects of Use of Cloned DNA problems. Each MCQ is crafted to challenge your understanding of Use of Cloned DNA principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Use of Cloned DNA MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Use of Cloned DNA MCQs | Page 4 of 8

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Q31.
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q32.
If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.

a.

N terminal

b.

C terminal

c.

Both N & C terminal

d.

Middle

Discuss
Answer: (a).N terminal
Q33.
Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________

a.

hybrid protein

b.

fusion protein

c.

combination protein

d.

insert protein

Discuss
Answer: (b).fusion protein
Q34.
It is also possible to generate a fusion protein in which ______ is encoded by the vector.

a.

N terminal

b.

C terminal

c.

Both N and C terminal

d.

Middle region

Discuss
Answer: (b).C terminal
Q35.
Which of the following is present in E. coli rrnB operon for rRNA?

a.

Initiation codon

b.

Termination codon

c.

Both initiation and termination codon

d.

Both are absent

Discuss
Answer: (c).Both initiation and termination codon
Q36.
Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector.

a.

origin, replication

b.

end, replication

c.

end, stability

d.

origin, stability

Discuss
Answer: (d).origin, stability
Q37.
In fusion proteins, the reading frame in a vector is out of phase with the insert.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q38.
Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.

a.

point mutations, decreases

b.

point mutations, increases

c.

frameshift mutations, increases

d.

frameshift mutations, increases

Discuss
Answer: (b).point mutations, increases
Q39.
Lambda PL promoter is used in which vectors?

a.

Cloning vectors

b.

Expression vectors

c.

Both cloning and expression vectors

d.

Bacteriophage Mu

Discuss
Answer: (b).Expression vectors
Q40.
The promoter can be controlled by a repressor which is temperature sensitive.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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